Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU.
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Purified PilB, PilT and PilU have ATPase activity; NTPase motif residues are needed for twitching. FimT is not mentioned in the abstract.
"Assay of purified 6xHis-tagged PilB, PilT and PilU from P. aeruginosa showed that all three proteins have ATPase activities in vitro."
Identification of two genes with prepilin-like leader sequences involved in type 4 fimbrial biogenesis in Pseudomonas aeruginosa.
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fimT and fimU encode prepilin-like proteins; the fimT mutant is wild type, but FimT overexpression replaces FimU in fimbrial biogenesis.
"overexpression of the fimT gene was found to be able to functionally replace the lack of a fimU gene product, suggesting a subtle role in fimbrial biogenesis"
The molecular basis of FimT-mediated DNA uptake during bacterial natural transformation.
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Purified P. aeruginosa FimT binds dsDNA in vitro (EMSA), whereas P. aeruginosa FimU does not; the FimTPa D-region grafted onto Legionella FimT restores transformation in a Legionella fimT mutant.
"Interestingly, FimT from both species binds to DNA and the affinity appears to be within the same order of magnitude as L. pneumophila FimT."
Pseudomonas aeruginosa minor pilins are incorporated into type IV pili.
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The five P. aeruginosa minor pilins studied are FimU, PilV, PilW, PilX and PilE; FimT is not among them, so FimT incorporation into pili has not been tested in P. aeruginosa.
"including five conserved pilin-like proteins of unknown function (FimU, PilV, PilW, PilX, and PilE in Pseudomonas aeruginosa)"