Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Novel transcription coactivator complex containing activating signal cointegrator 1.
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Identified ASCC1/p50 as part of a nuclear ASC-1 complex required for AP-1, SRF, and NF-kappaB transactivation.
"these results suggest that the endogenous hASC-1 complex appears to play an essential role in AP-1, SRF, and NF-kappaB transactivation"
Gastrin activates paracrine networks leading to induction of PAI-2 via MAZ and ASC-1.
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Supports a context-specific transcriptional role for ASC-1/p50 in gastrin-triggered PAI-2/SERPINB2 induction.
"Silencing at least two subunits of ASC-1, p50 and p65, inhibited gastrin-stimulated PAI-2 expression, indicating that ASC-1 acts as a transcription activator complex to regulate transcription."
DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation.
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Establishes the ASCC complex as an ALKBH3-associated alkylation repair complex.
"Our data provide a molecular mechanism by which ALKBH3 collaborates with ASCC to maintain genomic integrity in a cell-type specific manner."
A proteome-scale map of the human interactome network.
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High-throughput interactome resource; useful for PPI context but not sufficient to define ASCC1 core molecular function.
"Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions."
Mutations in Subunits of the Activating Signal Cointegrator 1 Complex Are Associated with Prenatal Spinal Muscular Atrophy and Congenital Bone Fractures.
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Human ASCC1 loss-of-function variants disrupt neuromuscular development and support ASCC1 as a nuclear ASC-1 complex subunit.
"We report on recessive loss-of-function mutations in two genes (TRIP4 and ASCC1) that encode subunits of the nuclear activating signal cointegrator 1 (ASC-1) complex."
A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair.
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Defines a ubiquitin-dependent ASCC alkylation-damage pathway and places ASCC1 in the ASCC2/ASCC3-associated repair complex.
"Mass spectrometric analysis of ASCC2-associated proteins revealed the constitutive association of ASCC3 and ASCC1"
RNA ligase-like domain in activating signal cointegrator 1 complex subunit 1 (ASCC1) regulates ASCC complex function during alkylation damage.
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ASCC1 interacts with ASCC3, regulates ASCC complex recruitment during alkylation damage, and is required for resistance to alkylation damage.
"ASCC1 knockout through a CRISPR/Cas9 approach results in alkylation damage sensitivity in a manner epistatic with ASCC3."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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BioPlex AP-MS is useful large-scale interaction context but should not drive a generic protein-binding core annotation.
"Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks."
Multimodal cell maps as a foundation for structural and functional genomics.
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U2OS multimodal cell-map evidence is broad interaction/localization context rather than ASCC1-specific mechanistic evidence.
"Here we construct a global map of human subcellular architecture through joint measurement of biophysical interactions and immunofluorescence images for over 5,100 proteins in U2OS osteosarcoma cells."
The ASC-1 complex promotes translation initiation by scanning ribosomes.
Investigating the role of ASCC1 in the causation of bone fragility.
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In a SMABF2 patient and in hMSCs, loss/knockdown of ASCC1 suppresses osteoblast differentiation and increases adipogenesis, with reduced RUNX2/SERPINF1 expression and TGF-beta/SMAD3 signaling, identifying ASCC1 as a pro-osteoblastogenic and anti-adipogenic regulator.
Ribosomal collision is not a prerequisite for ZNF598-mediated ribosome ubiquitination and disassembly of ribosomal complexes by ASCC.
ASCC1 structures and bioinformatics reveal a novel helix-clasp-helix RNA-binding motif linked to a two-histidine phosphodiesterase.
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Crystal and SAXS structures define ASCC1 as a coupled KH-like (helix-clasp-helix) RNA-binding domain and a two-histidine phosphodiesterase domain; ASCC1 binds sequence-selectively to CGCG-containing RNA in a manner dependent on the KH GXXG motif.
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No phosphodiesterase activity was detected on a tested 2-5A substrate, and an atypical active-site histidine rotamer implies a noncanonical substrate, so a specific catalytic reaction for ASCC1 cannot be asserted.
Oxidative demethylation of 1-meA damaged DNA By ALKBH3
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Reactome event for ALKBH3/ASCC-dependent oxidative demethylation of 1-meA damaged DNA.
"The reversal of alkylating damage of dsDNA by ALKBH3 requires the presence of DNA helicase ASCC3, a component of the activating signal co-integrator complex"
Oxidative demethylation of 3-meC damaged DNA By ALKBH3
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Reactome event for ALKBH3/ASCC-dependent oxidative demethylation of 3-meC damaged DNA.
"The reversal of alkylating damage of dsDNA by ALKBH3 requires the presence of DNA helicase ASCC3, a component of the activating signal co-integrator complex"
Oxidative dealkylation of 1-EtA damaged DNA by ABH3
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Reactome event for ALKBH3/ASCC-dependent oxidative dealkylation of 1-etA damaged DNA.
"The reversal of alkylating damage of dsDNA by ALKBH3 requires the presence of DNA helicase ASCC3, a component of the activating signal co-activator complex"
ALKBH3 associated with ASCC1:ASCC2:ASCC3 binds alkylated dsDNA containing 3-meC
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Reactome complex event placing ASCC1:ASCC2:ASCC3 with ALKBH3 on 3-meC-containing alkylated DNA.
"ASCC3 is a part of ASCC1:ASCC2:ASCC3 activating signal co-integrator complex, which unwinds dsDNA, providing an appropriate substrate for ALKBH3"
ALKBH3 associated with ASCC1:ASCC2:ASCC3 binds alkylated dsDNA containing 1-meA
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Reactome complex event placing ASCC1:ASCC2:ASCC3 with ALKBH3 on 1-meA-containing alkylated DNA.
"ASCC3 is a part of ASCC1:ASCC2:ASCC3 activating signal co-integrator complex, which unwinds dsDNA, providing an appropriate substrate for ALKBH3"
ALKBH3 in complex with ASCC1:ASCC2:ASCC3 binds alkylated DNA containing 1-etA