"BST1 (Cre16.g662600), BST2 (Cre16.g663400), and BST3 (Cre16.g663450) (collectively BST1–3) are paralogous bestrophin-like genes"

"BST2 and BST3 were previously reported as LCI11 by Fang et al. (16). An alignment between the 3 Chlamydomonas bestrophin-like proteins showed that the proteins are >80% identical to one another"

"predicted that each BST protein had a chloroplast transit peptide and was likely to be a chloroplast membrane protein"

"the selective pore is positively charged, supporting the hypothesis that BST1–3 transport negatively charged ions"

"the cia5 mutant exhibited severely reduced expression of BST1 and BST3 under both CO2 conditions, a transcriptional pattern observed with other CCM genes"

"All 3 genes had increased transcript levels within 2 h after the switch to low CO2"

"This line showed the same localization pattern as BST3 under the constitutive PSAD promoter (Fig. 3C), and quantification of enrichment showed a 1.46-fold enrichment"

"All 3 BST-like proteins localized to the thylakoid membranes of the chloroplast (Fig. 3A), and this localization visibly extended into the thylakoid tubules of the pyrenoid"

"The BST3 transcript was not detected in bst3 (SI Appendix, Fig. S4B), and the BST3 protein was absent"

"This led us to think that BST1 or BST2 function might be redundant with BST3"

"These results indicate that BST1–3 play an important role in Ci uptake and fixation in low CO2 conditions in Chlamydomonas."

"We propose that BST1–3 are the transporters that bring HCO3− to CAH3 inside the thylakoid."

"The presence of AtVCCN1 decreases pmf in Arabidopsis, but the presence of the 3 BST proteins increases pmf in Chlamydomonas."

"Both LCIB and LCIC interact with LCI11 (Cre16.g663450), and LCIC also interacts with Cre16.g662600"

"LCI11 and Cre16.g662600 directly interact, and both also interact with another bestrophin-like protein, Cre16.g663400."

"In the current model, bicarbonate (HCO3−) is channeled into the thylakoid lumen by bestrophin-like proteins 1-3 (BST1-3)"

"BST4 may function as an HCO3− channel, like that proposed for BST1-3 (Mukherjee et al. 2019), but there are currently no data to support this hypothesis"

"In this study, we bring evidence that AtVCCN1 forms a voltage-dependent Cl− channel, is located in the thylakoid membrane"

"However, the striking phenotype of ΔPtBST1 mutants, which almost lacks in the LC-inducible CCM, clearly indicates that even the BST factors outside of the pyrenoid are vital components of the CO2-evolving machinery"