Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
ABC transporter-mediated release of a haem chaperone allows cytochrome c biogenesis.
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E. coli CcmA2B1C1 complex is needed for ATP-dependent release of holo-CcmE; without CcmA or CcmB, holo-CcmE stays in a dead-end complex with CcmC.
"In the absence of CcmA or CcmB, holo(haem)CcmE binds to CcmC in a stable dead-end complex, indicating high affinity binding of haem to CcmC."
Multiple phenotypic alterations caused by a c-type cytochrome maturation ccmC gene mutation in Pseudomonas aeruginosa.
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Of the P. aeruginosa ccm mutants tested, only ccmC completely lost cytochrome c. It also showed pleiotropic loss of pyoverdine, catalase, motility and rhamnolipid.
"Only in the case of the ccmC mutant was cytochrome c production totally abrogated."
Co-ordination of iron acquisition, iron porphyrin chelation and iron-protoporphyrin export via the cytochrome c biogenesis protein CcmC in Pseudomonas fluorescens.
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P. fluorescens ccmC mutant accumulates protoporphyrin IX and has reduced heme-catalase-mediated oxidative stress resistance.
"Significantly reduced resistance to oxidative stress mediated by haem-containing catalases was observed for the ccmC mutant."
Impaired maturation of the siderophore pyoverdine chromophore in Pseudomonas fluorescens ATCC 17400 deficient for the cytochrome c biogenesis protein CcmC.
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The pyoverdine defect of ccmC mutants is attributed to a lack of periplasmic oxidizing power.
"We suggest that the conversion of ferribactin to pyoverdine does not take place in the DeltaccmC mutant because of lack of oxidizing power in the periplasm."
Different residues in periplasmic domains of the CcmC inner membrane protein of Pseudomonas fluorescens ATCC 17400 are critical for cytochrome c biogenesis and pyoverdine-mediated iron uptake.
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Six-TM topology of CcmC; some periplasmic residues affect pyoverdine without affecting cytochrome c.
"A topological model for CcmC, based on the analysis of alkaline phosphatase fusions, predicts six membrane-spanning regions with three periplasmic loops."
Physical interaction of CcmC with heme and the heme chaperone CcmE during cytochrome c maturation.
The CcmC:heme:CcmE complex in heme trafficking and cytochrome c biosynthesis.
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The periplasmic WWD domain of E. coli CcmC binds heme directly.
"For the first time, evidence that the external WWD domain of CcmC interacts directly with heme is presented."
Structures of the CcmABCD heme release complex at multiple states.
Architecture of the Heme-translocating CcmABCD/E complex required for Cytochrome c maturation.
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CcmABCD flops heme across the membrane at the CcmBC interfaces; the CcmC WxWD motif positions heme for attachment to CcmE.
"Our data suggest that the complex flops a heme group from the inner to the outer leaflet at its CcmBC interfaces, driven by ATP hydrolysis at CcmA."
UniProt entry Q9I3N5 (CCMC_PSEAE)