Annotation inferences using phylogenetic trees
Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.
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Purified SltB (SltB1, PA4001) and soluble MltB both release anhydromuropeptides from insoluble peptidoglycan.
"Both enzymes produced the same three major soluble products which were identified as anhydromuropeptides based on ESI-MS analysis"
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SltB is the non-lipidated, soluble enzyme of the pair.
"The second, nonlabeled enzyme was overexpressed in soluble form and hence was named soluble lytic transglycosylase B (SltB)."
Interaction of penicillin-binding protein 2 with soluble lytic transglycosylase B1 in Pseudomonas aeruginosa.
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SltB1 binds PBP2 by affinity chromatography and SPR, via the N-terminal non-PB module of PBP2.
"Penicillin-binding protein 2 (PBP2) was identified as a binding partner"
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MltB is the lipoprotein; SltB1, SltB2 and SltB3 are soluble paralogs.
"One, MltB, is a lipoprotein, whereas three other homologues are produced in soluble form"
UniProt entry Q9HX24 (Q9HX24_PSEAE)