GLT8D1 source guide

PMID:38066107: human 52–371 soluble fusion; nine-donor hydrolysis screening differs from product-resolved UDP-galactose transfer. Figure S2 and Results disagree about GalNAc hydrolysis.

PMID:35198895: Methods uses immunopurified HA-tagged full-length protein, nucleotide-sugar donor and UDP-Glo, without an acceptor. Mutant-dependent UDP release supports catalytic compatibility but does not identify a glucosylated product.

PMID:34746377: full-length FLAG-tagged human WT versus variants; Golgi-marker microscopy and a coupled phosphate assay with UDP-galactose/GlcNAc. The assay does not identify product structure.

PMID:19946888: abstract-only cache; the target-specific supporting table remains inaccessible. Retained XLS-named failures are HTML responses.

The actual PAINT tree places Q68CQ7 at AN57/PTN002492649. Glucose transfer is asserted at ancestral AN0/PTN004435860 from the three bacterial waaO/waaJ experimental donors; Golgi activity is asserted at eukaryotic AN1/PTN001012071. The separately restricted bacterial LPS process is not the molecular-function assertion. These are phylogenetic judgments; the human Golgi self-donor is legitimate experimental grounding.

GO:0035250 descends from GO:0008378 and GO:0016757; its definition specifies UDP-galactose without fixing acceptor linkage. GO:0000139 entails GO:0016020, supporting the broad membrane TOO_GENERAL judgment while its HDA table remains inaccessible. The broad IEA is refined by MODIFY; no redundant NEW row is created. Existing machine identifiers, qualifiers and evidence remain literal. No process annotation is proposed from disease or migration phenotypes.

The evidence record preserves source URLs, input hashes, six raw contexts, product declarations and the bounded ancestry proof. Publication-cache availability and separately read originals are recorded distinctly.