RLCK185 (OsRLCK185, Q6I5Q6, Os05g0372100) - curation notes

Sources

• Deep research: RLCK185-deep-research-falcon.md (Falcon); used for full-text details not in cached abstracts.
• Cached abstracts: PMID:23498959, PMID:28111288, PMID:28371870, PMID:30418086, PMID:37976240, PMID:24750441 (PBL27). Full text: PMID:38158656.
• PMIDs verified via PubMed MCP search "OsRLCK185" (16 hits).

Identity

• RLCK subfamily VII Ser/Thr kinase; Arabidopsis counterpart PBL27 PMID:24750441.

Receptor step

• Identified as interactor of the Xoo effector Xoo1488 PMID:23498959.
• Silencing: PMID:23498959
• UniProt PTM: Ser-240/Thr-241/Thr-246 phosphorylated by CERK1 (deep research: triple mutant abolishes phosphorylation collectively).
• Also phosphorylated by OsSERK2 in the OsFLS2 complex PMID:38158656.

Substrates / MAPK link (rice, two independent groups)

• OsMAPKKKepsilon (OsMAPKKK24): PMID:28111288; PMID:28111288
• OsMAPKKK18 (and Y2H with OsMAPKKK11): PMID:28371870
• OsDRE2a: PMID:30418086
• OsRLK902-1/2 stabilise OsRLCK185 [PMID:37976240 abstract].
• Contrast with Arabidopsis: the PBL27-MAPKKK5 step is disputed (RLCK VII-4 redundancy); in rice the MAP3K link is supported by two independent labs with different MAP3K substrates.

Curation decisions

• GO:0002768 EXP -> MODIFY to GO:0002752 (consistent with ORYSJ/CERK1, ARATH/PBL27, module).
• Kinase MF rows (EXP, IBA, IEA x2, EC, Rhea) ACCEPT; core MF GO:0004674.
• ATP binding, plasma membrane (IDA, IEA) ACCEPT.
• NEW GO:0043410 positive regulation of MAPK cascade: passes participation (phosphorylates the initiating MAP3K) and comparator (PBL27 carries GO:0043410 in GOA).
• No protein binding rows in GOA for this gene.
• Module agreement: chitin_perception annoton pbl27_kinase = GO:0004674 at plasma membrane, process GO:0002752 - matches. Module names MAPKKK5 as target; rice substrates are OsMAPKKKepsilon/OsMAPKKK18 (MAPKKK11/18 are MAPKKK5 orthologs).