GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000122

AtSubP analysis

PMID:12410806

Characterization of Arabidopsis thaliana AtFKBP42 that is membrane-bound and interacts with Hsp90.

PMID:14517332

TWISTED DWARF1, a unique plasma membrane-anchored immunophilin-like protein, interacts with Arabidopsis multidrug resistance-like transporters AtPGP1 and AtPGP19.

PMID:15133126

Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters.

PMID:20971896

The ER-localized TWD1 immunophilin is necessary for localization of multidrug resistance-like proteins required for polar auxin transport in Arabidopsis roots.

PMID:35512423

Arabidopsis TWISTED DWARF1 regulates stamen elongation by differential activation of ABCB1,19-mediated auxin transport.

url:https://pmc.ncbi.nlm.nih.gov/articles/PMC452592/

Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters

TWD1 associates with the tonoplast and ABCC1/2 and modulates vacuolar uptake in a substrate-dependent manner.

"TWD1-HA-positive fractions overlap to similar extends with both plasma membrane (40 and 50% sucrose) and vacuolar fractions (30 and 40% sucrose"

url:https://oup.silverchair-cdn.com/article-minimal/6581225

https://oup.silverchair-cdn.com/article-minimal/6581225

TWD1 regulates stamen auxin flow, filament elongation and pollen maturation.

"both pollen viability and pollen number were significantly reduced in the tw1 and the abcb1abcb19 double mutant"

url:https://pmc.ncbi.nlm.nih.gov/articles/PMC207015/

TWISTED DWARF1, a Unique Plasma Membrane-anchored Immunophilin-like Protein, Interacts with Arabidopsis Multidrug Resistance-like Transporters AtPGP1 and AtPGP19

Native twd1 mutants have reduced leaf expansion and epinastic growth.

"Double mutant and twd1-1 show strongly reduced leaf expansion and strong epinastic growth behavior."

PMID:32699109

Auxin-transporting ABC transporters are defined by a conserved D/E-P motif regulated by a prolylisomerase.

DEP-motif mutagenesis links ABCB1 binding to TWD1-mediated activation.

"The D/E-P1008 motif is also important for ABCB1-TWD1 interactions and activation of ABCB1-mediated auxin transport by TWD1."

PMID:41027915

HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane.

TWD1 connects selected ABCB clients with HSP90-mediated membrane stability.

"serving as a co-chaperone of this ABC-FKBP-HSP90 dynamic module."

url:https://www.biorxiv.org/content/10.64898/2026.07.24.740493v2.full

The FKBP42, TWISTED DWARF1, prioritizes auxin over brassinosteroid transport by peptidyl-prolyl cis-trans isomerization of ABCB1

Recombinant FKBD1–180 shows CaM-dependent tetrapeptide isomerase activity in Figure5.

"Next, we measured the PPIase activity of recombinant FKBD (TWD11-180) protein"