A proteomics dissection of Arabidopsis thaliana vacuoles isolated from cell culture.

The study inventories purified vacuolar membrane and sap proteins; AFB2 target-level identification remains to be verified.

PMID:32067636

Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions.

AFB2 has both nuclear and cytoplasmic pools in root epidermal cells.

"AFB2 through AFB5 are distributed between the nucleus and the cytoplasm"

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

PMID:17410169

Mechanism of auxin perception by the TIR1 ubiquitin ligase.

TIR1 structural InsP6 provides the comparative basis for AFB2 cofactor binding.

"the leucine-rich repeat domain of TIR1 contains an unexpected inositol hexakisphosphate co-factor"

PMID:15992545

Plant development is regulated by a family of auxin receptor F box proteins.

PMID:20018756

Complex regulation of the TIR1/AFB family of auxin receptors.

Native genetics establish strong TIR1/AFB2 contributions in seedling roots.

"TIR1 and AFB2 are the dominant auxin receptors in the seedling root"

PMID:22466420

A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin.

AFB2 participates in auxin-dependent, partner-selective co-receptor interactions.

"IAA12 interacted specifically with TIR1 and AFB2 at 100 μM IAA"

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000122

AtSubP analysis

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000041

Gene Ontology annotation based on UniPathway vocabulary mapping

file:ARATH/AFB2/AFB2-uniprot.txt

AFB2-uniprot.txt

PMID:21828251

miR393 and secondary siRNAs regulate expression of the TIR1/AFB2 auxin receptor clade and auxin-related development of Arabidopsis leaves.

AFB2 transcript regulation provides receptor-abundance control.

PMID:25604443

SCFTIR1/AFB-based auxin perception: mechanism and role in plant growth and development.

Summarizes SCF-mediated auxin perception; receptor biochemical diversity is assessed with primary studies.

PMID:36289340

Adenylate cyclase activity of TIR1/AFB auxin receptors in plants.

Available AC experiments do not directly establish AFB2 activity.

file:publications/url_https___pure.coventry.ac.uk_ws_portalfiles_portal_60931587_Post_Print.pdf.md

Adenylate cyclase activity of TIR1/AFB auxin receptors in plants (author-accepted manuscript)

Expression failure limits interpretation for AFB2.

"Due to lack of detectable protein, the AC activity of the other 4 TIR1/AFBs could not be assessed."

file:interpro/panther/PTHR16134/PTHR16134-paint.tsv

PANTHER PTHR16134 PAINT ancestral assertions

The receptor-specific and broader F-box assertions have distinct ancestral placements.