Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin superfamily peptides and their receptors in the common octopus (Octopus vulgaris).
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OPR was cloned from O. vulgaris and shown to be a GPCR of the OT/VP receptor superfamily
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OPR is specifically activated by octopressin but not cephalotocin or vertebrate OT/VP peptides
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Functional assay in Xenopus oocytes showed calcium-mediated inward chloride current upon OP stimulation
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Key ligand-binding residues conserved in vertebrate OT/VP receptors are substituted in OPR
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OPR mRNA is expressed in both the nervous system and peripheral tissues
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OP induces contractile activity in rectum, oviduct, efferent branchial vessel, spermatophoric gland, and anterior aorta
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OPR gene has an intron between TM6 and TM7, consistent with OT/VP receptor gene structure
Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily.
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First octopus OT/VP receptor (CTR1) cloned, specific for cephalotocin
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CTR1 is not activated by octopressin, establishing ligand specificity
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CTR1 expression in nervous system and reproductive tissues
An Octopus-Derived Peptide with Antidiuretic Activity in Rats.
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Cephalotocin but not octopressin acts as a selective agonist of human AVP V1b and V2 receptors
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Confirms the distinct pharmacological profiles of octopressin versus cephalotocin
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Octopressin shows no activity on human OT/VP receptors, indicating its binding pocket is uniquely adapted to OPR
Comparative and Evolutionary Physiology of Vasopressin/Oxytocin-Type Neuropeptide Signaling in Invertebrates.
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OPR expression in buccal and gastric ganglia is indicative of a role in regulation of feeding and digestion
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OPR is expressed in renal appendages, reproductive ducts (oviduct, vas deferens), and branchial tissues in addition to the nervous system
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Octopressin/OPR system handles primarily peripheral physiological challenges (osmoregulation, metabolism under stress), complementing the cephalotocin system
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In cuttlefish, octopressin affects memory formation, enhancing long-term memory at low dose but impairing it at higher dose, suggesting central neuromodulatory roles
Osmotic/ionic status of body fluids in the euryhaline cephalopod suggest possible parallel evolution of osmoregulation.
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Octopressin injection in O. ocellatus causes significant decrease in hemolymph osmolarity and reduced Ca2+ and Na+ in blood and urine within 24 hours
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Cephalotocin injections had no impact on fluid balance, confirming octopressin alone mediates osmoregulatory function through OPR
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Octopressin enables hyperosmotic regulation in euryhaline octopus species facing dilute seawater