Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
UniProtKB O00522: human KRIT1 sequence and curated functions
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In human KRIT1, the FERM domain mediates Rap1A/Rap1B binding and is needed for PIP2 binding.
"The FERM domain mediates binding to RAP1A and RAP1B and is"
UniProtKB A0A9L0SR44: horse KRIT1 sequence and domains
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A0A9L0SR44 belongs to PANTHER subfamily PTHR13283:SF11, the subfamily that contains human KRIT1.
"PTHR13283:SF11; KREV INTERACTION TRAPPED PROTEIN 1"
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A0A9L0SR44 is the product of a non-canonical Ensembl transcript of the horse KRIT1 gene.
"ENSECAT00000146800.1; ENSECAP00000077417.1; ENSECAG00000016850.4"
KRIT1 human-horse sequence comparison
KRIT1 re-review: horse KRIT1 gene products vs human O00522
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A0A9L0SR44 is identical to the 736-aa horse KRIT1 product A0A3Q2GZV2 except for 17 absent C-terminal residues and a divergent five-residue end.
"| A0A3Q2GZV2 | A0A9L0SR44 | 736 | 719 | 716/719 | 99.6% | 0 | 17 |"
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In A0A9L0SR44, the human HEG1-groove leucines L717 and L721 have no aligned residue, while the Rap1-interface residue R452 is retained.
"A715->gap, L717->gap, L721->gap, P731->gap"
A mechanism of Rap1-induced stabilization of endothelial cell--cell junctions.
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KRIT1 is a GTP-dependent, Rap1-specific effector. Rap1 binding releases KRIT1 from microtubules so that it can move to junctions.
"KRIT1 is an authentic Rap1-specific effector that binds strongly to the effector loop of active Rap1A"
Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1.
Cerebral cavernous malformation protein CCM1 inhibits sprouting angiogenesis by activating DELTA-NOTCH signaling.
CCM1 regulates vascular-lumen organization by inducing endothelial polarity.
Mechanism for KRIT1 release of ICAP1-mediated suppression of integrin activation.
KRIT1 regulates the homeostasis of intracellular reactive oxygen species.
Structural basis of the junctional anchorage of the cerebral cavernous malformations complex.
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The HEG1 tail binds a groove at the KRIT1 FERM F1/F3 interface. KRIT1(L717,721A) loses HEG1 affinity and does not target to endothelial junctions.
"This structure enabled design of KRIT1(L717,721A), which exhibited a >100-fold reduction in HEG1 affinity."