Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
YeastPathways to GO-CAM conversion
The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization.
Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway.
High confidence proteomic analysis of yeast LDs identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation.
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.
Enzymatic properties of squalene epoxidase from Saccharomyces cerevisiae.
Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles.
SGD gene description for ERG1 (yeast)
Regulated targeting of the monotopic hairpin membrane protein Erg1 requires the GET pathway.
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Get3 recognises the hairpin targeting element of Erg1, and the GET pathway is especially needed for acute upregulation of Erg1 under low-sterol conditions.
"Get3 recognizes the hairpin targeting element of Erg1 via its classical client-binding pocket."
Lipid saturation induces degradation of squalene epoxidase for sterol homeostasis and cell survival.
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Lipid saturation in ole1 mutants triggers Doa10-Ubc7-dependent ERAD of Erg1; the P469L mutation abolishes this degradation.
"Using these mutants, we show that lipid saturation triggers the endoplasmic reticulum-associated degradation (ERAD) of squalene epoxidase Erg1, a rate-limiting enzyme in sterol biosynthesis, via the E3 ligase Doa10-Ubc7 complex."
Reengineering the Substrate Tunnel to Enhance the Catalytic Efficiency of Squalene Epoxidase.
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Yin et al. 2024 engineered S. cerevisiae Erg1 variants that increased squalene epoxidase catalytic activity and ergosterol titer, reinforcing the assigned squalene monooxygenase activity.
"the catalytic activity of the squalene epoxidase from Saccharomyces cerevisiae was significantly improved by reshaping its substrate tunnel"