Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Acetyl-Coenzyme A acyltransferase 2 attenuates the apoptotic effects of BNIP3 in two human cell lines.
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Identifies ACAA2 as a BNIP3 binding partner (membrane yeast two-hybrid, pull-down, co-IP) that co-localizes with BNIP3 in mitochondria and abolishes BNIP3/hypoxia-induced apoptosis in HepG2 and U-2 OS cells, linking fatty acid metabolism to apoptosis.
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
The crystal structure of human mitochondrial 3-ketoacyl-CoA thiolase (T1): insight into the reaction mechanism of its thiolase and thioesterase activities.
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Crystal structures (apo and CoA-bound, 2.0 A) confirm ACAA2/T1 is a tetrameric degradative thiolase; demonstrates significant biosynthetic (acetoacetyl-CoA) thiolase activity and low intrinsic acyl-CoA thioesterase (hydrolase) activity, fastest for butyryl-CoA. Provides the EXP/IDA basis for the thiolase and hydrolase molecular-function annotations.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Cloning and sequence analysis of a full length cDNA encoding human mitochondrial 3-oxoacyl-CoA thiolase.
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Original cDNA cloning of human mitochondrial 3-oxoacyl-CoA thiolase (397 aa, 86.6% identity to rat), expressed in liver, fibroblasts and muscle. Establishes gene identity and mitochondrial assignment; makes no claim about cholesterol biosynthesis.
ACAA2 tetramer transfers acyl group from Ac-CoA to acyl-CoA forming 3OA-CoA and CoA-SH