Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Mitochondrial aspartate aminotransferase ( maa1 ) inactivation causes glutamate-requiring glu1 mutation in Schizosaccharomyces pombe.
Cytosolic aspartate aminotransferase encoded by the AAT2 gene is targeted to the peroxisomes in oleate-grown Saccharomyces cerevisiae.
Autophagy sustains glutamate and aspartate synthesis in Saccharomyces cerevisiae during nitrogen starvation.
UniProt entry O42652 (caa1)
The cytosolic form of aspartate aminotransferase is required for full activation of TOR complex 1 in fission yeast.
-
Native caa1 disruption causes aspartate auxotrophy; catalytic-site substitution impairs growth/TORC1 response, without establishing direct TOR binding or sensing.
"Deletion of the entire ORF of caa1 + from the genome by replacing it with KanMX (Δ caa1 ) resulted in cells that showed the expected slow growth, aspartate auxotrophy, and rapamycin sensitivity"