ndk (PA3807, Q59636) review notes

Literature

Search (Europe PMC REST, 2026-11-05: TITLE:"nucleoside diphosphate kinase" AND aeruginosa, 30 hits read as titles). Papers used (all abstract only):

  1. Kavanaugh-Black et al. 1994 PMID:8016083: Ndk purified from P. aeruginosa; activity
    also associated with succinyl-CoA synthetase.
  2. Shankar et al. 1996 PMID:8606147: a 16-kDa cytoplasmic form and a protease-derived
    12-kDa membrane-associated form; both autophosphorylate; the membrane form prefers GTP
    synthesis PMID:8606147.
  3. Sundin et al. 1996 PMID:8809750: cloning of ndk; NDK activity in cytosolic and
    membrane fractions; cytosolic Ndk transfers the ATP gamma-phosphate to other NDPs
    non-specifically PMID:8809750. ndk
    hyperexpression in an algR2 mutant restored alginate to ~60% of wild type
    PMID:8809750. The authors link this to GTP supply for GDP-mannose.

UniProt Q59636 (reviewed): HAMAP MF_00451, active-site His117 (phosphohistidine).

Alginate: AlgA (GDP-mannose pyrophosphorylase) uses GTP; Ndk supplies GTP, a co-substrate,
and performs no step of alginate synthesis. The algR2 (AlgQ) mutant is a regulatory
mutant affecting many genes, and the rescue is partial. This is a necessity/precursor
supply phenotype, not participation.

PseudoCAP IBA-seed assessment

PTN node node term n_seeds leaf IBAs seed annotation (term, evidence, PMID) verdict rationale other seeds IBD stands without this seed?
PTN000887110 GO:0004550 nucleoside diphosphate kinase activity 21 412 (137 taxa) GO:0004550, IDA, PMID:8809750 (PseudoCAP) SOUND Abstract reports NDK activity assays in cytosolic and membrane fractions and the substrate range of the cytosolic enzyme; HAMAP NDK with active-site His. E. coli Ndk (P0A763), human NME1-4 (P15531, P22392, Q13232, O00746), mouse/rat Nme, yeast YNK1, S. pombe ndk1, Arabidopsis NDPK1/2, Drosophila awd, Dictyostelium, zebrafish, Plasmodium, maize Yes; NDK is among the best characterised enzymes and the node is richly seeded. No GO_CENTRAL defect.

Annotation decisions