ARO10 notes

Identity

• YDR380W; ThDP/Mg-dependent 2-oxo-acid decarboxylase of the PDC family [file:yeast/ARO10/ARO10-uniprot.txt "Note=Binds 1 thiamine pyrophosphate per subunit."]
• Pyruvate is not a substrate [file:yeast/ARO10/ARO10-uniprot.txt "low efficiency substrate and transaminated valine and pyruvate are no"]; PMID:22904058

Evidence

• Physiological phenylpyruvate decarboxylase PMID:12902239
• Redundancy with PDC1/5/6 for Phe/Trp PMID:12499363; minor in leucine in that study PMID:12499363
• Isoleucine PMID:10753893
• Methionine PMID:16423070
• Recombinant enzyme: efficient aromatic 2-keto acid decarboxylase PMID:21501384, but questions BCAA/Met role PMID:21501384
• Counterpoint (in cell extracts, Km): PMID:22904058
• Post-transcriptional control / possible partner PMID:15933030

Curation decisions

• REMOVE pyruvate decarboxylase (IBA from PDC clade; IEA from RHEA:54360 which is actually the 4-methyl-2-oxopentanoate reaction).
• nucleus IBA: over-annotation.
• generic catalytic / carboxy-lyase: MODIFY to specific decarboxylase terms.
• Core: phenylpyruvate decarboxylase (Phe catabolism), indolepyruvate decarboxylase (Trp), branched-chain 2-oxoacid decarboxylase (Leu/Ile).

Module notes (ehrlich_pathway)

• ARO10 is the committed decarboxylase; PDC1/5/6 contribute redundantly for BCAA/aromatics; THI3 is not an active decarboxylase (regulatory).
• YeastPathways leucine pathway row assigns EC 4.1.1.43 (phenylpyruvate decarboxylase) to the leucine reaction - mapping quirk; EC 4.1.1.72 is the appropriate one.