Mutation of active site serine residue with cysteine displays change in acyl-acceptor preference of β-peptidyl aminopeptidase from Pseudomonas aeruginosa PAO1.
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Recombinant BapF (PA1486) hydrolyses beta-homoGly-pNA and performs aminolysis; Ser237 is the catalytic residue and S237C switches the acyl acceptor from water to amines.
"Wild-type BapF showed βhGly-βhGly-pNA synthetic activity, but the product vanished in a few minutes and converted to free βhGly."