This guide locates the literature and database evidence underlying the review. A normal cache's full_text_available flag describes its acquisition result; the actual inspected sections and unresolved limits are stated separately below. Cached files retain their original bytes and flags. Reference-only supports identify evidence without adding quotations.
289fb901b3eac205c2de6b3163c2284467d2fe9ae06f1f6e6809da7b90f04f94. Main Results covering alpha homodimer/tetramer/decamer behavior and the Materials and Methods sections on purification, analytical ultracentrifugation and nucleotide exchange were read (saved extracted lines 1–260 and 410–615). Human subunits were expressed in E. coli; human eIF2 was produced in yeast. No supplemental insert sequences or figure pixels were independently analyzed. Alpha enhances assembled-complex exchange, while alpha-free activity remains possible.42a3f61b2f06be491e32214aef9cd7dc175370bc50fb437c3d9e80cdfb27ca5a; Methods passage 12 and Results passage 23 locate the human comparison among all 27 inspected passages. Detailed recombinant structural work concerns S. pombe. The human endpoint is translation, not isolated-alpha exchange.The reproducible database analysis gives exact source-row joins, full selected MITAB fields, reference checksum comparisons, independent partner-class sources and target-specific reaction chains. Its source manifest records input URLs and hashes; its archive contains database records, not article bodies.
For PMID:25416956, the original two-hybrid screening/retesting description was read, including quadruplicate retesting and identity confirmation. Orthogonal assays validate a subset of the map, not necessarily each EIF2B1 pair. For PMID:25502805 and PMID:31515488, operative original Methods distinguish wild-type positive pairs, mutation checks, autoactivation controls and repeated disruption tests. For PMID:29892012, original Methods/Results distinguish experimentally positive wild-type pairs from computational predictions. For PMID:32296183, inspected Results/Discussion and literal target records support the positive pairs; the normal cache does not expose complete Methods, and pair-specific physiological context or orthogonal retests are not inferred.
GO_REF:0000033 assertions are traced to four literal IBD entries at PTN000028064 in PTHR45860. The human target inherits that node and exactly matches the 305-aa sequence in the alignment. A single human-target donor for initiation-factor activity is legitimate grounding, not circularity. The other computational references describe annotation methodology and are assessed as background rather than independent target experiments.
Rat Q64270 response premises are visible in the complete captured QuickGO response. PMID:9341116 reports whole-complex response to heat in rat cells, PMID:8567668 reports glucose-related exchange activity in rat islets, and PMID:8430778 reports eIF2B regulation in diabetic rat muscle. Complete abstracts and the curated donor assertions were inspected; operative full Methods were not recovered. These are conserved complex-response contexts, not proof of autonomous alpha sensing or a human experiment.
PMID:12499492 is an authentic clinical report with only its abstract accessible here; no negative inference is drawn from its EIF2B5-focused title. The PMID:15217090 original was inspected in selected Methods, Results and Discussion windows on autopsy specimens, genotypes, immunostaining, quantification and oligodendrocyte outcomes. Alpha-specific participation remains unresolved. PMID:8626696's publisher source supplied indexed Results/Discussion on human T-cell activation, whole-complex activity and beta abundance; complete Methods and the alpha-specific T-cell-receptor-signaling assertion remain unresolved. These three contexts are UNDECIDED rather than declared false or misattributed.
Paths below are relative to the repository root. Cache full text reports the preserved acquisition flag, not a claim that every original section was read. The same PMID retains its fetched identity and title.
| Source | Cache full text | Actual reading and limit |
|---|---|---|
| PMID:8626696(https://pubmed.ncbi.nlm.nih.gov/8626696/) | false | Abstract plus selected original Results and Discussion. Recovered publisher-indexed operative text reports primary human T-cell activation, whole eIF2B exchange and beta-subunit abundance. Complete Methods and EIF2B1-specific receptor-signaling participation remain unresolved; foregrounding beta is not a negative alpha experiment. |
| PMID:9235896(https://pubmed.ncbi.nlm.nih.gov/9235896/) | false | Selected original construct, binding and imaging sections. Recovered author original identifies human cDNA, mouse receptor tails, GST pull-down and tagged-human imaging in 293 cells. Historical M1/M14/M20 engineered starts are not the natural 222-aa product. Historical and current curated tail coordinates are retained source-specifically. Full paper and all image pixels were not independently inspected. |
| PMID:11323413(https://pubmed.ncbi.nlm.nih.gov/11323413/) | false | Abstract and independent corroboration. Authentic complete abstract read; publisher full route failed. Positive alpha omission/add-back and complex function are retained. Species and exact construct details of the original preparation are not independently resolved. |
| PMID:11835386(https://pubmed.ncbi.nlm.nih.gov/11835386/) | false | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic abstract reports mutations in all five eIF2B subunit genes, including one alpha-subunit patient. It supports EIF2B1 disease relevance but not a specific oligodendrocyte-development mechanism or natural-isoform equivalence. |
| PMID:12499492(https://pubmed.ncbi.nlm.nih.gov/12499492/) | false | Abstract only. Authentic clinical abstract and publication identity read. Publisher full route returned 403; source-specific EIF2B1 heat support remains unresolved. The EIF2B5-focused title is not used to allege a wrong-gene annotation. |
| PMID:14702039(https://pubmed.ncbi.nlm.nih.gov/14702039/) | false | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic FLJ abstract establishes a large-scale human cDNA resource; UniProt links this source to isoform 2 sequence. No alternative-product functional equivalence follows, and the original clone table was not independently re-read. |
| PMID:15060152(https://pubmed.ncbi.nlm.nih.gov/15060152/) | true | Selected original Methods and Results plus cached Discussion. The normal cache is partial despite its full_text_available flag. Indexed PMC recovery supplies cloning of full-length human subunits, sequencing, complex-formation Results and Figure 1 description. Not all original sections or figure pixels were inspected. |
| PMID:15217090(https://pubmed.ncbi.nlm.nih.gov/15217090/) | false | Selected original Methods, Results and Discussion. Author-uploaded original text windows cover autopsy specimens, genotypes, immunostaining, quantification and oligodendrocyte outcomes. These do not resolve alpha-specific participation. Figure captions were read, not figure pixels. Publisher failure is preserved. |
| PMID:15489334(https://pubmed.ncbi.nlm.nih.gov/15489334/) | false | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic MGC abstract describes sequence-resource generation and quality. This is sequence provenance and background, not an independent EIF2B1 mechanistic experiment. |
| PMID:15776425(https://pubmed.ncbi.nlm.nih.gov/15776425/) | false | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic abstract identifies EIF2B1 Val183Phe among reported patient variants. Exact assay consequences are not inferred from mutation ascertainment alone. |
| PMID:16289705(https://pubmed.ncbi.nlm.nih.gov/16289705/) | false | Abstract and independent corroboration. Authentic abstract reports eIF2/eIF2B supplementation of a HeLa translation system. DOI retrieval did not provide an operative full body. Exact preparation details are not inferred from the cell-extract host. |
| PMID:16541075(https://pubmed.ncbi.nlm.nih.gov/16541075/) | false | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic abstract describes the finished human chromosome 12 sequence. It supports genomic provenance, not a molecular-function or localization experiment on EIF2B1. |
| PMID:19608861(https://pubmed.ncbi.nlm.nih.gov/19608861/) | false | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic abstract describes a global lysine-acetylation survey. EIF2B1-specific peptide spectra or regulatory consequences were not independently reanalyzed; no function is inferred from modification detection. |
| PMID:21269460(https://pubmed.ncbi.nlm.nih.gov/21269460/) | true | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic abstract describes a seven-human-cell-line central-proteome dataset. No target-specific detection table or functional consequence is independently claimed here. |
| PMID:22814378(https://pubmed.ncbi.nlm.nih.gov/22814378/) | true | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic abstract describes NatB substrate profiling and functional tests on other proteins. Official EIF2B1 processing/modification provenance does not establish a distinct functional product. |
| PMID:24275569(https://pubmed.ncbi.nlm.nih.gov/24275569/) | false | Authentic abstract; target mechanistic/table evidence not reanalyzed. The authentic abstract describes a human-liver phosphoproteomics dataset. No EIF2B1-specific phosphosite consequence or mechanism is asserted from the general survey. |
| PMID:25416956(https://pubmed.ncbi.nlm.nih.gov/25416956/) | true | Original selected Results plus literal pair records. Independently read saved original paragraphs 23–24: two screens, fresh-colony quadruplicate pairwise retesting, at least three positives and confirmed identity. Orthogonal validation is dataset-level unless a specific pair is documented; no EIF2B1-specific orthogonal result is invented. |
| PMID:25502805(https://pubmed.ncbi.nlm.nih.gov/25502805/) | true | Operative original cached Methods and Results. Read entries 15,40,69,80,95–96, including wild-type positive selection, verified human ORFeome clones, autoactivation controls and repeated Y2H comparisons. Target variants and positive baseline are resolved from literal IntAct records. |
| PMID:25858979(https://pubmed.ncbi.nlm.nih.gov/25858979/) | false | Original main text and captions; supplementary Methods unavailable. Original main text and captions were inspected, not supplementary Methods. Whole-complex GDP exchange and CHO Eif2b4 mutants support complex regulation, not autonomous alpha catalysis. The normal cache remains abstract-only. |
| PMID:27023709(https://pubmed.ncbi.nlm.nih.gov/27023709/) | true | Complete indexed operative Methods and Results. Read BioC passages 0–26. Detailed expression/structure concerns S. pombe; Methods passage 12 and Results passage 23/Figure 2h include the human factor comparator in reconstituted translation. This readout is not an isolated-alpha exchange assay. |
| PMID:29599213(https://pubmed.ncbi.nlm.nih.gov/29599213/) | true | Original main Results and Methods. Author-laboratory PDF read through extracted lines 1–260 and 410–615: human subunit cloning, alpha homodimer, tetramer/decamer reconstitution, GDP-exchange and purification methods. No SI sequences or figure-pixel analysis. Human eIF2 was expressed in yeast; assay host does not change construct species. |
| PMID:29892012(https://pubmed.ncbi.nlm.nih.gov/29892012/) | true | Original operative Methods and Results. Read BioC passages 10,11,13,27,45–46,61,142. Wild-type strong Y2H pairs, empty-vector autoactivation control and mutant comparisons are distinct from computational predictions. GORASP2 positive/no-effect record is source-specific; no orthogonal EIF2B1 retest is asserted. |
| PMID:31048492(https://pubmed.ncbi.nlm.nih.gov/31048492/) | false | Abstract and structural-resource records. Operative article Methods were not recovered. EMDB/PDB data identifies two canonical human 305-aa alpha chains; current RIKEN plasmid sequence independently matches that product but is not proof of the exact historical experimental batch. |
| PMID:31515488(https://pubmed.ncbi.nlm.nih.gov/31515488/) | true | Operative original cached Methods. Read entries 61,64,73–74,81: full-clone mutation checks, wild-type positive binding, autoactivation controls and three-screen disruption criterion. PCA uses a subset; the specific EIF2B1 pairs are not claimed to be orthogonally retested. |
| PMID:32296183(https://pubmed.ncbi.nlm.nih.gov/32296183/) | true | Original cached Results and Discussion plus literal pair records. Read entries 17,43,45: quadruplicate pairwise retesting and sequence confirmation, dataset-level orthogonal validation, and limits on cellular context/isoform coverage. The normal cache does not expose complete Methods. Positive target pairs are literal IntAct joins; no physiological mechanism is transferred from partners. |
| PMID:9341116(https://pubmed.ncbi.nlm.nih.gov/9341116/) | false | Complete abstract and curated rat donor annotation; operative full Methods unavailable. Whole-complex regulation is distinguished from isolated alpha activity. |
| PMID:8567668(https://pubmed.ncbi.nlm.nih.gov/8567668/) | false | Complete abstract and curated rat donor annotation; operative full Methods unavailable. Whole-complex regulation is distinguished from isolated alpha activity. |
| PMID:8430778(https://pubmed.ncbi.nlm.nih.gov/8430778/) | false | Complete abstract and curated rat donor annotation; operative full Methods unavailable. Whole-complex regulation is distinguished from isolated alpha activity. |
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The selected GOA records at physical lines 35–36 assign UniProt enables to GO:0005085 with PMID:25858979 and PMID:27023709 (annotation date 2023-10-11). These bytes remain unchanged. Official GO relation guidance distinguishes an activity executed by a product from its contribution to a complex activity not attributable to that subunit. The review records a probable WRONG_QUALIFIER query, with proposed contributes_to, unknown cause and NOT_REPORTED status. It preserves the biological GEF contribution and does not assert a confirmed database error.
The source distinction is auditable at the public locators above: PMID:25858979 main paragraphs 5–12 and Figure 1C–G/Figures 2–3 captions describe whole-complex exchange and delta-mutant experiments; supplementary Methods remain inaccessible. PMID:27023709 Methods passage 12 and Results passage 23/Figure 2h identify the human translation comparator. PMID:29599213 Results on alpha homodimer/complex assembly, Figure 1G–I captions and Methods on human subunit cloning provide independent alpha contribution evidence; the original extracted text at lines 195–248 and 410–525 distinguishes that assembly role from the catalytic subcomplex. No supplemental insert sequence or figure-pixel claim is added.
The existing source-access entries for PMID:8626696, PMID:12499492 and PMID:15217090 remain operative. Their cited sibling-review summaries do not resolve the source-specific alpha uncertainties. PMID:31048492 remains abstract/deposition evidence; the proposed phospho-eIF2alpha question seeks adequate subunit-specific assay/contact evidence without presenting missing access as a biological knowledge gap. The natural 222-aa product comparison is a proposed experiment, not an observed result.