Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

PMID:12643545

Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.

Identifies RPN1 among proteins in early melanosome fractions by mass spectrometry; RPN1 is an abundant ER protein and a likely ER contaminant of the melanosome preparation.

PMID:15835887

Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits.

Ribophorin I is present in all isolated mammalian OST subcomplexes, supporting its membership in the OST complex and contribution to its glycotransferase activity.

PMID:17081065

Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.

Detects RPN1 in melanosome proteomes across developmental stages; consistent with ER-protein co-purification rather than a melanosome-specific role.

PMID:17264154

Ribophorin I acts as a substrate-specific facilitator of N-glycosylation.

RNAi depletion of ribophorin I dramatically reduces N-glycosylation of selected membrane proteins; RPN1 acts as a chaperone/escort presenting substrates to the catalytic STT3 subunits, and is not essential for N-glycosylation per se.

PMID:19167329

Cotranslational and posttranslational N-glycosylation of polypeptides by distinct mammalian OST isoforms.

Ribophorin I depletion co-reduces both STT3A and STT3B and lowers OST activity, placing RPN1 in a shared complex with the two catalytic isoforms.

PMID:19946888

Defining the membrane proteome of NK cells.

High-throughput MS membrane-proteome dataset in which RPN1 is detected; supports only a generic membrane localization.

PMID:22658674

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.

Global mRNA-interactome-capture screen assigning RPN1 as an RNA-binding protein; likely reflects its cytoplasmic contact with the translating ribosome rather than sequence-specific RNA binding.

PMID:22988243

Malectin forms a complex with ribophorin I for enhanced association with misfolded glycoproteins.

RPN1 forms a stable complex with the ER lectin malectin, enhancing association with misfolded glycoproteins; RPN1 may act as a chaperone recognizing misfolded proteins.

PMID:25416956

A proteome-scale map of the human interactome network.

High-throughput Y2H interactome mapping RPN1 interactions (e.g. SGTA, UBQLN1).

PMID:27974209

TMEM258 Is a Component of the Oligosaccharyltransferase Complex Controlling ER Stress and Intestinal Inflammation.

TMEM258 is a required OST component essential for N-linked glycosylation; RPN1 and TMEM258 form STT3A subcomplex 1, consistent with the recorded RPN1-TMEM258 interaction.

PMID:30021884

Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.

Crosslinking-MS dataset recording RPN1 interactions with OST partners including STT3A, STT3B and malectin.

PMID:31515488

Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.

Interactome-variant screen recording RPN1 interactions (SGTA, UBQLN1).

PMID:31831667

Cryo-electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B.

Cryo-EM structures assign RPN1 as a subunit of both OST-A and OST-B; in OST-A its cytoplasmic region forms a ribosome-binding four-helix bundle, disordered in OST-B.

PMID:32296183

A reference map of the human binary protein interactome.

Binary interactome (HuRI) recording RPN1 interactions (SGTA, CAMLG, KRTAP1-3).

PMID:32707033

Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.

Kinase-interaction network recording an RPN1-POMK interaction.

PMID:35271311

OpenCell: Endogenous tagging for the cartography of human cellular organization.

Endogenous-tagging interactome recapitulating RPN1 associations with STT3A, STT3B and malectin, consistent with OST membership.

PMID:36697828

Visualization of translation and protein biogenesis at the ER membrane.

In situ cryo-ET visualization of the OST complex (including RPN1) as part of the OST-A assembly at the translating ER membrane.

PMID:38670073

Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB.

CRISPR/cryo-EM characterization of the OST-A (STT3A) complex, with RPN1 modeled as an OST-A subunit; OST-A is required for TLR4 N-glycosylation and NF-kB signaling.

PMID:9642163

Interleukin-2 induces N-glycosylation in T-cells: characterization of human lymphocyte oligosaccharyltransferase.

Purification of human lymphocyte OST identified ribophorin I (with ribophorin II and a Wbp1 homolog) as a predominant subunit of the N-glycosylation enzyme.

Reactome:R-HSA-446209

Transfer of N-glycan to the protein

Reactome:R-HSA-9694793

Spike protein gets N-glycosylated

Reactome:R-HSA-9816276

CDH1 is N-glycosylated on asparagine residues in endoplasmic reticulum

Reactome:R-HSA-9918962

E is N-glycosylated

Reactome:R-HSA-9918988

pre-M is N-glycosylated

Reactome:R-HSA-9919011

Pre-NS1 folds and is N-glycosylated

Reactome:R-HSA-9931286

CD274 N-linked glycosylation in ER