CFAP43 is an axonemal structural protein. The native human respiratory-axoneme model in PMID:37258679(https://doi.org/10.1038/s41586-023-06140-2), human structure section and model-building Methods, identifies the conserved tether/tether-head apparatus. Official PDBe 8J07 entity 81 maps chains i6/i8 to Q8NDM7, residues 1–1665. This connects experimentally constrained structure to the human target. It does not mean every residue was resolved or every alternative product was tested. The canonical-sequence model is distinguished from the five preserved UniProt products.
The detailed algal Results show FAP43 integrated with FAP44 in a structural assembly. PMID:29514928(https://doi.org/10.1091/mbc.E18-02-0142), Figure 1 and cryo-ET Results, independently removes the tether/tether-head in Tetrahymena fap43 mutants while I1 dynein remains visible. Algal FAP244 redundancy explains why many Chlamydomonas fap43 repeats retain this structure. PMID:29687140(https://pubmed.ncbi.nlm.nih.gov/29687140/), native-tag and waveform Results, retains cilia number/length but changes waveform in Tetrahymena. Neither organism is a direct human construct assay. The detailed prestroke map in PMID:39743588(https://doi.org/10.1038/s41586-024-08337-5) is bovine sperm; its CFAP43/CFAP44 helices participate in the inner-arm docking network. Dynein motor properties are not CFAP43 enzymatic activities.
These results support one NEW molecular function, GO:0005200 structural constituent of cytoskeleton, and one integrated structural core. All 13 original assertions are retained. Existing sperm axoneme assembly includes organization as well as initiation: PMID:29449551(https://doi.org/10.1038/s41467-017-02792-7), Figures 2 and 4–5, demonstrates human and mouse sperm architectural defects; the mouse figure describes the axoneme as “fully disorganized”. Respiratory cilia need not fail to form. PMID:31884020(https://doi.org/10.1016/j.ydbio.2019.12.010), selected primary Results, tracheal imaging Methods and Movie S4/S5 legends, instead separates altered mouse beat frequency from reduced fluid transport. The indexed primary text was accessible, although a subsequent direct publisher opening returned 403 and the normal cache remains abstract-only. No complete PDF or every figure was read.
A source-identity issue affects the preserved ISS axoneme row: UniProt Q57WH1 is Trypanosoma CFAP44/Tb927.7.3560; the original paper's CFAP43 is Q580P9/Tb927.4.5380. Both were actually localized to axonemes. Retaining correct target biology is supported by that CFAP43 experiment and the independent native human structure. The original WITH field remains exact; the historical transfer decision is unresolved. The asymmetric Trypanosoma doublet localization relative to its paraflagellar rod is not automatically transferred to human cilia.
The PAINT source PTN008557012, original donors, and self-evidence are preserved. The full ancestral node/alignment history was not reconstructed. A target's own experimental annotation can ground a phylogenetic inference and is not circular. Current mouse E9Q7R9 and Ensembl protein identifiers agree, and exact donor GOA records support the location, motility, frequency and sperm-assembly transfers. The original ARBA rule was not reconstructed, but independent primary movement evidence is available.
CFAP43-source-evidence.json contains literal donor identity and GOA fields, exact human PDBe mapping, typed is_a/part_of ontology receipt, source URLs/hashes and selected reading locators. It is an evidence extract, not an additional biological experiment. GO-CAM's exact source index has no target match. No new process is proposed; no process-hierarchy/comparator exception or invented tether-complex GO term is needed. GO:0005200 is a molecular-function addition, independently supported by the target structure.
The original seven reference identity/title pairs and all five products remain unchanged. Supplemental references use genuine normal caches; existing main caches for PMID:37258679 and PMID:39743588 are byte-preserved. PMID:28552195 remains abstract-only and corroborative. PMID:31004071 is background clinical/ciliary context, not a new activity or a basis for claiming one universal clinical syndrome. Short distinct primary excerpts are counted across YAML and this new notes file; there is no inherited notes prefix for this new review.
Normal research was attempted with Falcon (90-second timeout), then the explicit perplexity-lite fallback (HTTP 401 insufficient quota). No provider report was produced or invented. Manual primary research supplies the scientific evidence above. Applicable campaign instructions are pinned in the published project. Artifact status is derived by the normal tools; campaign completion awaits the approved, checked publication merge.
Open mechanistic questions concern product-specific incorporation and separation of structural docking from beat control. Product-matched rescue and structure-guided interface mutants in human airway cultures could measure incorporation, waveform, frequency and transport separately. These are prospective experiments, not results or claims of CFAP43 motor activity.
This section supersedes any implication that every accepted source term must be repeated verbatim in the compact core summary. It preserves all 13 source assertions and their ACCEPT actions, plus the existing structural molecular-function NEW.
The frequency assignment is now explicit in the integrated structural core. It rests on complementary observations: native human CFAP43-containing tether architecture (PMID:37258679); actual Tetrahymena fap43 loss displacing retained I1 motor domains in the Results/Figure 4 of PMID:29514928; and mouse tracheal frequency/flow changes with grossly retained respiratory cilia (PMID:31884020). This is a conserved mechanistic synthesis, not an assertion that the proposed algal powerstroke model alone proves human frequency regulation. The precise human frequency-setting interface remains unestablished. The separate algal fap44 kinase/phosphorylation experiment is not relabeled as a CFAP43 assay.
Flagellated-sperm motility remains a core manifestation of CFAP43's own work in the moving axoneme, even when its loss also disrupts assembly. The mouse sperm experiment does not separate beat-specific from architectural failure. Current official is_a/part_of ancestry places GO:0030317 under the core GO:0003341, so no duplicate process is needed in the core list. Motile cilium remains an accepted enclosing core compartment; its broader granularity relative to 9+2 cilium or axoneme is not grounds for a non-core label on this original assertion. No new annotation process is proposed.
The structural MF retains enables: CFAP43 itself makes a structural contribution within the assembled apparatus. This does not imply that it acts alone or has the motor activity of dynein. A curator-facing question now asks whether a dedicated tether/tether-head complex term would be appropriate; no unsupported complex identifier is assigned.
The PMID:31884020 cache remains abstract-only. The saved authentic publisher-indexed primary response includes the tracheal Methods and Movie S4/S5 legends, which state the direction of the frequency and flow differences. Neither the actual videos nor a complete PDF were inspected. Citation verification and selected external access are documented separately from cache completeness; a blanket full-text-unavailable assertion would conceal the actual accessed sections. The durable source artifact records the original URL, response hash and section locators.
All original source objects, qualifiers, partners, products and reference identities are retained. The complete previous notes are preserved above. This append introduces no literature quotations; the additional five-word PMID:29514928 YAML anchor keeps its aggregate current-YAML excerpts below 25 words. Existing inherited notes quotations remain separately disclosed.