TLN (talin, Oscarella pearsei, UniProt A0A3G2LGI8) -- review notes

Automated deep research was unavailable for this gene (no deep-research provider
keys in this environment). These notes were written manually from the cached full
text of the only paper on this protein (PMID:29880641, Miller et al. 2018 J Biol
Chem), which is primarily about the sponge vinculin VIN1, and from the UniProt
Swiss-Prot entry (TLN_OSCPE).

Identity and domains

• 2531 aa talin; conserved talin architecture: FERM head, talin rod with
  vinculin-binding sites, C-terminal I/LWEQ actin-binding domain
  PMID:29880641.
• Has an extra predicted PTB domain after the FERM domain
  PMID:29880641.
• InterPro: Talin_cent, VBS, I/LWEQ, FERM, Talin1/2 VBS2, IBS2B; PANTHER PTHR19981.

Experimental data

• The ONLY experiment on TLN: a synthetic peptide corresponding to TLN residues
  598-621 (homologous to the mouse talin vinculin-binding site 605-628) was made
  and used in ITC and actin co-sedimentation with recombinant VIN1
  PMID:29880641.
• The peptide binds VIN1 (full-length and D1) and activates VIN1 F-actin binding
  PMID:29880641;
  PMID:29880641.
• Full-length TLN protein was never expressed; TLN localization, integrin binding,
  actin binding and membrane binding were NOT examined in sponge.

Family background (as stated in PMID:29880641)

• PMID:29880641
• PMID:29880641

Curation decisions (summary)

• protein binding IPI (with VIN1) -> MODIFY to vinculin binding GO:0017166
  (peptide-level evidence).
• IEA localization/MF rows accepted on family-conservation grounds, except:
  ruffle and structural constituent of cytoskeleton (MARK_AS_OVER_ANNOTATED),
  cell-cell adhesion (MODIFY to cell-matrix adhesion, the integrin-linked context).
• No NEW annotations: no sponge-specific experimental evidence beyond the VBS peptide.