DYNC2LI1 (Q8TCX1) research notes

Cytoplasmic dynein 2 light intermediate chain 1 (D2LIC, LIC3), human.

Deep research status

Falcon deep research succeeded (DYNC2LI1-deep-research-falcon.md; the first attempt with the 600 s default timed out, and the rerun with --timeout 2400 succeeded). Its summary agrees with the publication-based review below. The review's supporting quotes come from the cached publications.

Summary of function

• Identified as a D2LIC that binds DHC2. PMID:11907264 It was originally seen at the Golgi PMID:11907264.
• Part of the retrograde IFT motor across species. PMID:12802074
• Structural role: PMID:31451806. The Ras-like core has a conserved nucleotide-binding pocket (PMID:31451806).
• Loss of function (SRTD15 patient fibroblasts, RPE1 knockdown): [PMID:26077881 "We show that DYNC2LI1 is essential for dynein-2 complex stability, is expressed in the cartilage growth plate, and plays critical roles in the regulation of primary cilium length, retrograde IFT, and Hedgehog signaling."; "Our results demonstrate that loss of DYNC2LI1 markedly impairs retrograde IFT and leads to the accumulation of IFT proteins within the primary cilium."]
• Localization: PMID:26130459

Key curation decisions

• Core MF: dynein heavy chain binding (IBA + IDA). Contributes to minus-end-directed motor activity as a non-catalytic subunit.
• REMOVE: male germ cell nucleus (NAS; the cited paper does not mention the gene). Also protein binding with SOD1 (generic, BAG3 paper).
• MODIFY: protein binding (DYNC2H1) changed to dynein heavy chain binding.
• MARK_AS_OVER_ANNOTATED: colocalizes with cytoplasmic microtubule (ISS).
• Golgi (EXP 2002), centrosome, transition zone and regulation of cilium assembly (length phenotype) are KEEP_AS_NON_CORE.

HPA cilium atlas vs module role

• Module stage 4: dynein-2 subunit; complex-level MF minus-end-directed MT motor activity; retrograde IFT; axoneme.
• HPA v25: Primary cilium (Supported), Basal body (Supported); main locations Cytosol and Mid piece. GOA carries the HPA rows (GO_REF:0000052) for cilium, ciliary basal body and cytosol, all reviewed (cilium/basal body ACCEPT, cytosol non-core).
• Assessment: HPA agrees with the module role (cilium plus ciliary base pool). core_functions uses contributes_to minus-end-directed MT motor activity, in cytoplasmic dynein complex, for retrograde IFT. No disagreement.