Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
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CaMK Ser/Thr kinase functions (intracellular signal transduction, Ca2+/CaM- dependent protein kinase activity, calmodulin binding, calcium-dependent kinase activity) are propagated across the PANTHER phylogenetic group for this protein.
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
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SwissProt keyword-derived (SPKW) annotations present in the Sept 2025 goa_uniprot_gcrp snapshot but removed from the current GOA release after GOA retired the keyword2GO pipeline for cellular organisms.
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For rice CCAMK the keyword "Nodulation" mapped to GO:0009877; rice is a non-nodulating cereal, so this is a pathway/organism-context over-annotation inherited from legume CCaMK/DMI3 orthologs. The genuine rice symbiotic process is AM symbiosis.
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Fungal symbiosis in rice requires an ortholog of a legume common symbiosis gene encoding a Ca2+/calmodulin-dependent protein kinase.
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Demonstrates that rice OsCCaMK is the ortholog of the legume common-symbiosis gene DMI3/CCaMK and is required for arbuscular mycorrhizal (fungal) symbiosis in rice.
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OsCCaMK is not induced by mycorrhization but is genetically required for it; Osccamk mutants are Myc-defective.
OsDMI3 is a novel component of abscisic acid signaling in the induction of antioxidant defense in leaves of rice.
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OsDMI3 was experimentally localized to the nucleus, the cytoplasm and the plasma membrane.
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ABA, H2O2 and PEG induce OsDMI3 expression and kinase activity; H2O2 is required for the ABA-induced increases under water stress.
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OsDMI3 is required for ABA-induced increases in the activities of the antioxidant enzymes SOD and CAT; it is an important component of ABA-induced antioxidant defense in rice.
Deep-research report (falcon / Edison Scientific Literature) - functional annotation of rice CCAMK / OsCCaMK / OsDMI3 (Q6AVM3).
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Confirms identity - rice OsCCaMK/OsDMI3 = Os05g0489900 = UniProt Q6AVM3, a Ca2+/CaM-dependent Ser/Thr protein kinase with an N-terminal kinase domain, a CaM- binding/autoinhibitory region and C-terminal EF-hand Ca2+-binding motifs.
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Establishes the core role - CCaMK decodes nuclear Ca2+ spiking in the common symbiosis signaling pathway (CSSP), phosphorylating CYCLOPS (with DELLA) to activate RAM1 and arbuscule development; in rice it is genetically required for AM symbiosis and Osccamk mutants are Myc-defective.
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Notes rice is a non-nodulating cereal; the kinase's symbiotic competence for nodulation is shown only heterologously (rice OsCCaMK restores nodulation in legume dmi3/ccamk mutants), so "nodulation" is not a rice process.
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Documents the separable abiotic-stress role - OsDMI3 in ABA-ROS signaling, phosphorylating OsRBOHB to promote H2O2 and potentiate ABA, and acting upstream of OsMPK1; also implicated in saline-alkaline tolerance in roots.
UniProtKB entry Q6AVM3 (CCAMK_ORYSJ) - downloaded Swiss-Prot record for rice OsCCaMK / OsDMI3.
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Annotates an N-terminal protein-kinase domain (residues 13-298) with an ATP- binding region (BINDING 19..27) and ATP-binding residue 43, three C-terminal EF-hand Ca2+-binding domains, and a calmodulin-binding region (321-334).
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CATALYTIC ACTIVITY records EC 2.7.11.17 phosphorylation of L-seryl-[protein] (RHEA:17989) and L-threonyl-[protein] (RHEA:46608), the source of the Rhea-derived protein serine kinase activity annotation.