Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds.
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PA14 transposon mutants in each cbc gene, including PA5376, fail to grow on choline, betaine and carnitine as sole carbon sources.
"The PA5376::tn, PA5377::tn and PA5378::tn mutants all failed to grow on choline (Fig. 3A) and betaine (Fig. S2) as sole C sources"
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CbcV is the ATPase of the CbcWV core transporter, which works with at least three SBPs.
"In addition to the core transporter comprised of a transmembrane protein (CbcW) and an ATPase (CbcV), the Cbc transporter system interacts with at least three SBPs"
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Carnitine uptake by CbcWV requires the separately encoded SBP CaiX (PA5388).
"These findings provide compelling evidence that Psyr_2916 and PA5388, hereafter referred to as CaiX, enable Cbc-mediated carnitine uptake in P. syringae and P. aeruginosa respectively."
Roles of three transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa choline uptake for catabolism.
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CbcXWV is one of three choline transporters able to support growth on choline; its expression is induced by GbdR and glycine betaine.
"BetT3 and CbcXWV, but not BetT1, were sufficient to support growth on choline."
The widespread plant-colonizing bacterial species Pseudomonas syringae detects and exploits an extracellular pool of choline in hosts.
Differential requirements for processing and transport of short-chain versus long-chain O-acylcarnitines in Pseudomonas aeruginosa.
UniProt entry Q9HTI8 (PA5376)