Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The gene for an exopolyphosphatase of Pseudomonas aeruginosa.
Exopolyphosphatase of Pseudomonas aeruginosa is essential for the production of virulence factors, and its expression is controlled by NtrC and PhoB acting at two interspaced promoters.
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ppx null mutant phenotypes in motility, pigment, AHLs, rhamnolipids and biofilm.
"Results obtained from the ppx null mutant demonstrated that Ppx is involved in the production of virulence factors associated with both acute infection (e.g. motility-promoting factors, blue/green pigment production, C6-C12 quorum-sensing homoserine lactones) and chronic infection (e.g. rhamnolipids, biofilm formation)."
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Dual NtrC/sigma54 and PhoB/sigma70 promoters.
"Under nitrogen-limiting conditions, its expression was controlled from a σ(54)-dependent promoter activated by the response regulator NtrC."
Pseudomonas aeruginosa Exopolyphosphatase Is Also a Polyphosphate: ADP Phosphotransferase.
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Purified paPpx hydrolyses polyP to polyPn-1 + Pi.
"Pseudomonas aeruginosa exopolyphosphatase (paPpx; EC 3.6.1.11) catalyzes the hydrolysis of polyphosphates (polyP), producing polyPn-1 plus inorganic phosphate (Pi)."
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paPpx also has polyP:ADP phosphotransferase activity at the same active site.
"We show that paPpx is also a polyphosphate:ADP phosphotransferase and that the active site is the same as that one involved in the hydrolase activity."
UniProt entry Q9ZN70 (PPX_PSEAE)