The research report should be a detailed narrative explaining the function, biological processes, and localization of the gene product. Citations should be given for all claims.
You should prioritize authoritative reviews and primary scientific literature when conducting research. You can supplement
this with annotations you find in gene/protein databases, but these can be outdated or inaccurate.
We are specifically interested in the primary function of the gene - for enzymes, what reaction is catalyzed, and what is the substrate specificity? For transporters, what is the substrate? For structural proteins or adapters, what is the broader structural role? For signaling molecules, what is the role in the pathway.
We are interested in where in or outside the cell the gene product carries out its function.
We are also interested in the signaling or biochemical pathways in which the gene functions. We are less interested in broad pleiotropic effects, except where these elucidate the precise role.
Include evidence where possible. We are interested in both experimental evidence as well as inference from structure, evolution, or bioinformatic analysis. Precise studies should be prioritized over high-throughput, where available.
The target gene cryaa in Danio rerio (zebrafish) encodes αA‑crystallin, a member of the small heat shock protein (sHSP/HSP20) family that functions as an ATP‑independent molecular chaperone in the ocular lens. Zebrafish Cryaa is reported as a 173 aa protein with ~72% amino‑acid identity to human CRYAA, consistent with the UniProt description and α‑crystallin/sHSP domain family membership. (rossen2025zebrafishasa pages 3-4)
α‑Crystallins are sHSPs that bind destabilized proteins and inhibit their aggregation, supporting proteostasis in the unusually protein‑dense lens environment. (zou2015aconservedrole pages 1-2, slingsby2013evolutionofcrystallins pages 1-2)
A mechanistic theme emphasized in authoritative reviews is that α‑crystallins form large, dynamic oligomers, and subunit exchange/oligomer dynamics are needed for chaperone function—a property that also helps avoid crystallization/phase separation at high protein concentration in the lens. (slingsby2013evolutionofcrystallins pages 1-2, rossen2025zebrafishasa pages 2-3)
Lens fiber cells eliminate organelles during maturation; as a result, lens transparency depends heavily on maintaining crystallin solubility and preventing aggregation. α‑Crystallin chaperone activity is implicated in supporting protein turnover/quality control in these organelle‑free fiber cells. (rossen2025zebrafishasa pages 3-4)
Across zebrafish literature, cryaa is described as lens‑restricted at the tissue level, with embryonic expression reported in lens epithelial and fiber cells. (rossen2025zebrafishasa pages 3-4, peng2024thegenerationand pages 2-2)
Single‑cell transcriptomic evidence summarized in a zebrafish lens/cataract review indicates cryaa is exclusive to lens fiber cells and is the earliest expressed crystallin, beginning by ~48 hours post‑fertilization (hpf) and increasing over the next ~3 days. (rossen2025zebrafishasa pages 3-4)
Within the retrieved primary literature set, I did not find direct experimental evidence specifying Cryaa’s subcellular localization (e.g., cytosolic vs membrane‑bound fractions, nuclear association) in zebrafish lens cells; the evidence supports lens‑cell type localization rather than intracellular compartment localization. (rossen2025zebrafishasa pages 3-4)
The zebrafish cryaa promoter is widely used as a lens‑specific driver:
- In Peng et al. 2024 (Jan 2024; Molecular Vision), a Tol2 transgenic line zTg(cryaa:Cre‑cryaa:EGFP) showed Cre/EGFP expression in the lens, with reported onset of Cre/EGFP expression beginning at ~22 hpf, and high lens recombination efficiency (~98.4% in a reported assay). (peng2024thegenerationand pages 1-2, peng2024thegenerationand pages 6-7)
- In Posner et al. 2019 (Mar 2019; PLOS ONE), the αA‑crystallin promoter drove strong GFP expression in the cloche lens, supporting utility for lens-targeted expression experiments. (posner2019whydoesthe pages 1-2)
A strong zebrafish in vivo link between αA‑crystallin function and client solubility comes from the cloche mutant lens cataract model:
- In cloche lenses, γ‑crystallins become insoluble and lenses show marked opacity/reflectance; overexpression of exogenous αA‑crystallin (cryaa) solubilized γ‑crystallin, increased transparency, and promoted fiber differentiation. (goishi2006αacrystallinexpressionprevents pages 1-2, goishi2006αacrystallinexpressionprevents pages 6-7)
Quantitative client/solubility evidence:
- γ‑crystallin solubility fractions: WT 83% soluble / 17% insoluble, cloche 20% soluble / 80% insoluble, and cloche + αA overexpression >60% soluble γ‑crystallin. (goishi2006αacrystallinexpressionprevents pages 6-7)
These results support a zebrafish‑context “client class” for Cryaa: lens crystallins (notably γ‑crystallins) that otherwise undergo insolubilization/aggregation. (goishi2006αacrystallinexpressionprevents pages 1-2, goishi2006αacrystallinexpressionprevents pages 6-7)
In transgenic zebrafish models expressing cataract‑linked αA‑crystallin mutants under the cryaa promoter (lens‑specific), R49C but not R116C promoted aggregation of a destabilized human γD‑crystallin mutant in the lens, indicating mutation-specific disruption of chaperone/client interactions in vivo. (wu2018transgeniczebrafishmodels pages 1-2)
Zou et al. 2015 (Sep 2015; Experimental Eye Research) concluded that αA‑crystallin has a conserved role in zebrafish embryonic lens development and that genetic loss produces lens abnormalities including increased reflectance (reduced transparency), with a more consistent and severe phenotype in maternal/zygotic mutants compared with morpholino knockdown. (zou2015aconservedrole pages 1-2, zou2015aconservedrole pages 8-9)
Rescue evidence:
- Lens phenotype penetrance was reduced by transgenic expression of rat αA‑crystallin, and severity was attenuated by maternal αA‑crystallin. (zou2015aconservedrole pages 1-2, zou2015aconservedrole pages 8-9)
The most direct quantitative evidence is contained in the paper’s table/figures retrieved as images (Table 1; Figures 2–4), including penetrance by genotype and quantitative reflectance scoring. (zou2015aconservedrole media a1406ec4, zou2015aconservedrole media d5eb8994, zou2015aconservedrole media bc004cf0, zou2015aconservedrole media a21c2392)
A zebrafish review summarizing multiple cryaa mutant studies reports that:
- ~60% of cryaa−/− embryos from homozygous crosses show variable lens defects by ~72 hpf in one CRISPR line.
- Another report observed >90% penetrance in homozygous crosses, attributed in part to maternal transcript transmission and/or background effects.
- Baseline cataract frequencies differ by strain (e.g., AB ~16% at 96 hpf vs TL ~9% at 96 hpf), emphasizing that penetrance comparisons require strain context. (rossen2025zebrafishasa pages 3-4)
Goishi et al. 2006 (Jul 2006; Development) provides unusually rich quantitative phenotyping:
- Cataract/opacity penetrance in cloche at 2.5 dpf: 68% (428/633) cloudy; by 3 dpf penetrance rises (reported 84% (533/633) in one analysis and 100% (34/34) in another). (goishi2006αacrystallinexpressionprevents pages 4-5)
- Lens reflectance: median reflectance intensity was 37‑fold higher in cloche vs WT at 4 dpf (P=0.0209). (goishi2006αacrystallinexpressionprevents pages 4-5)
- αA‑crystallin protein was reduced ~85% by Western blot in cloche. (goishi2006αacrystallinexpressionprevents pages 4-5)
Rescue by αA overexpression (cryaa):
- 80.2% of embryos avoided cataract formation by bright‑field scoring.
- Lens reflectance was reduced by 41%.
- γ‑crystallin insolubility was reduced by 60%.
- Fiber‑cell nuclei per section (4 dpf): WT 0.0±0.0, cloche 22.6±5.0, and cloche + αA 3.0±0.0 (mean±s.d.). (goishi2006αacrystallinexpressionprevents pages 6-7)
Collectively, this supports that Cryaa contributes to lens transparency both by maintaining client crystallin solubility and by supporting normal fiber differentiation/denucleation under stress/pathological contexts. (goishi2006αacrystallinexpressionprevents pages 6-7)
Wu et al. 2018 (Nov 2018; PLOS ONE) used a 1.2 kb zebrafish cryaa promoter for lens‑specific expression of mutant αA‑crystallins and reported:
- In a cryaa‑null background, penetrance approached ~100% for αA‑R49C and ~80% for αA‑R116C.
- A destabilized γD‑crystallin I4F mutant alone produced ~30% frequency of major lens defects.
- Lens defects first visible at ~3 dpf; phenotypes scored at 4 dpf using defined severity classes. (wu2018transgeniczebrafishmodels pages 4-5, wu2018transgeniczebrafishmodels pages 2-4)
Authoritative review framing emphasizes that α‑crystallins are sHSP chaperones whose dynamic oligomeric assembly supports both chaperone function and the structural requirement of avoiding protein condensation in the lens. (slingsby2013evolutionofcrystallins pages 1-2)
In zebrafish, Cryaa is explicitly framed as supporting protein turnover/quality control in organelle‑free fiber cells via chaperone activity. (rossen2025zebrafishasa pages 3-4)
A major 2024 advance with direct translational relevance is the discovery that an E3 ligase (RNF114) can promote UPS‑dependent degradation of aggregated/mutant CRYAA:
- Yang et al. 2024 (Sep 2024; J Clin Invest; https://doi.org/10.1172/jci169666) report that clearance of mutant CRYAA aggregates is blocked by proteasome inhibition (MG132) but not by autophagy/lysosome inhibitors, supporting a proteasome‑dependent mechanism. (yang2024reversiblecoldinducedlens pages 2-3)
- They engineered a deliverable RNF114 complex/peptide that reduced lens opacity in rodent models and was also effective in H2O2‑induced zebrafish cataract models, demonstrating a concrete zebrafish application for CRYAA‑targeted proteostasis therapy development. (yang2024reversiblecoldinducedlens pages 8-10, yang2024reversiblecoldinducedlens pages 1-2)
Zebrafish enables:
- Rapid in vivo scoring of lens transparency/reflectance (e.g., cloche reflectance quantification; mutant penetrance scoring). (goishi2006αacrystallinexpressionprevents pages 6-7, goishi2006αacrystallinexpressionprevents pages 4-5)
- Lens‑specific transgenesis using cryaa promoter to express pathogenic variants and to test client aggregation mechanisms (e.g., γD‑crystallin aggregation with αA mutants). (wu2018transgeniczebrafishmodels pages 2-4, wu2018transgeniczebrafishmodels pages 1-2)
The zTg(cryaa:Cre‑cryaa:EGFP) line provides a lens‑restricted Cre driver for conditional genetic manipulation in zebrafish lenses, with reported early lens expression and high Cre activity (98.4% in one assay) and minimal developmental impact. (peng2024thegenerationand pages 1-2, peng2024thegenerationand pages 6-7)
cloche cataract model (Goishi 2006; Development; 2006-07):
- Opacity penetrance: 68% (428/633) at 2.5 dpf; 84% (533/633) at 3 dpf; also reported 100% (34/34) in one analysis. (goishi2006αacrystallinexpressionprevents pages 4-5)
- Lens reflectance: 37-fold higher in cloche vs WT at 4 dpf (P=0.0209). (goishi2006αacrystallinexpressionprevents pages 4-5)
- Cryaa rescue: 80.2% prevention of cataract; 41% reduction in reflectance; 60% reduction in γ-crystallin insolubility; nuclei/section reduced from 22.6±5.0 to 3.0±0.0. (goishi2006αacrystallinexpressionprevents pages 6-7)
cryaa mutant penetrance and timing (summary across studies):
- cryaa expression begins ~48 hpf; defects visible by ~72 hpf. (rossen2025zebrafishasa pages 3-4)
- cryaa−/− defect penetrance reported ~60% in one setting and >90% in another; baseline cataracts differ by strain (AB 16% at 96 hpf; TL 9% at 96 hpf). (rossen2025zebrafishasa pages 3-4)
cataract-linked mutant transgenics (Wu 2018; PLOS ONE; 2018-11):
- αA-R49C: ~100% penetrance in cryaa−/− background; αA-R116C: ~80% penetrance in cryaa−/− background. (wu2018transgeniczebrafishmodels pages 4-5)
- γD I4F alone: ~30% major defect frequency. (wu2018transgeniczebrafishmodels pages 4-5)
Primary molecular function: ATP‑independent sHSP/α‑crystallin holdase chaperone that binds destabilized lens proteins and suppresses aggregation to maintain lens proteostasis and optical transparency. (zou2015aconservedrole pages 1-2, slingsby2013evolutionofcrystallins pages 1-2)
Primary biological processes: lens development and maintenance of transparency (anti‑aggregation), supporting fiber‑cell differentiation/denucleation especially under stress/pathological conditions. (zou2015aconservedrole pages 1-2, goishi2006αacrystallinexpressionprevents pages 6-7)
Localization (best-supported): lens tissue (lens epithelial/fiber cells; fiber-cell enriched by scRNA summary); intracellular compartment not resolved in the retrieved zebrafish evidence. (rossen2025zebrafishasa pages 3-4)
Key pathways: lens proteostasis network; recent work highlights UPS-mediated turnover of aggregated CRYAA as a therapeutic axis (RNF114). (yang2024reversiblecoldinducedlens pages 8-10)
The following table consolidates the zebrafish cryaa functional annotation and evidence types.
| Category | Key findings (1-2 sentences) | Key evidence/citations |
|---|---|---|
| Molecular function | Zebrafish-specific: cryaa encodes αA-crystallin, a small heat shock protein/α-crystallin family member with ATP-independent chaperone activity in the lens. General α-crystallin context: α-crystallins bind thermodynamically destabilized proteins and help prevent aggregation; oligomer/subunit exchange is important for chaperone function. | (zou2015aconservedrole pages 1-2, rossen2025zebrafishasa pages 3-4, slingsby2013evolutionofcrystallins pages 1-2, rossen2025zebrafishasa pages 2-3) |
| Clients/targets | Zebrafish-specific: in the cloche lens, loss/insolubility of αA-crystallin is associated with γ-crystallin insolubility, and exogenous αA-crystallin can solubilize γ-crystallin. General α-crystallin context: destabilized lens proteins/crystallins are canonical client classes for α-crystallin chaperones. | (goishi2006αacrystallinexpressionprevents pages 1-2, zou2015aconservedrole pages 9-9) |
| Biological processes | Zebrafish-specific: cryaa supports embryonic lens development, lens transparency, and lens fiber-cell differentiation/denucleation. General context: α-crystallins contribute to proteostasis in organelle-free fiber cells where protein turnover/refolding capacity is limited. | (zou2015aconservedrole pages 1-2, goishi2006αacrystallinexpressionprevents pages 1-2, rossen2025zebrafishasa pages 3-4) |
| Cellular/tissue localization | Zebrafish-specific: cryaa expression is lens-restricted at the tissue level and is reported in lens epithelial and fiber cells; single-cell transcriptomics further indicate cryaa is exclusive to lens fiber cells in embryos/larvae. No direct subcellular localization of Cryaa protein within lens cells was retrieved from the provided evidence. | (rossen2025zebrafishasa pages 3-4, peng2024thegenerationand pages 2-2) |
| Developmental expression timing | Zebrafish-specific: cryaa is among the earliest crystallins expressed, detectable by ~48 hpf and increasing over the next 3 days; cryaa-promoter transgenes can begin lens expression earlier, with cryaa:Cre/EGFP activity reported from 22 hpf and zygotic transgene activity noted around 16 hpf in one 2024 tool paper. | (rossen2025zebrafishasa pages 3-4, peng2024thegenerationand pages 1-2, peng2024thegenerationand pages 6-7, peng2024thegenerationand pages 7-8) |
| Phenotypes (LOF) | Zebrafish-specific: cryaa loss causes variable embryonic lens defects, including increased lens reflectance/opacity, roughness at primary fiber cells or peripheral fiber-cell boundaries, central pitting, abnormal fiber-cell interfaces, and mild delay of denucleation; αA-crystallin increases the probability/severity of defects but is not absolutely essential for lens formation. Maternal cryaa contributes to phenotypic buffering. | (zou2015aconservedrole pages 1-2, zou2015aconservedrole pages 8-9, rossen2025zebrafishasa pages 4-5, rossen2025zebrafishasa pages 3-4, zou2015aconservedrole media a1406ec4) |
| Phenotypes (mutant overexpression) | Zebrafish-specific: lens-targeted expression of cataract-linked αA-crystallin mutants (e.g., R49C, R116C) produces lens abnormalities, with stronger effects for R49C and greater defect frequency when combined with cryaa-null background. R49C also promotes aggregation of destabilized γD-crystallin in vivo, supporting a client-trapping/aggregation mechanism. | (rossen2025zebrafishasa pages 4-5, rossen2025zebrafishasa pages 3-4) |
| Quantitative data points | Zebrafish-specific: reported penetrance includes ~60% of cryaa−/− embryos from homozygous crosses showing lens defects, and >90% penetrance in another homozygous-cross setting attributed partly to maternal effects; baseline cataract frequency varies by strain (AB ~16% at 96 hpf, TL ~9% at 96 hpf; ZDR ~27% by 18 months). Adult zebrafish lens protein composition includes ~8% α-crystallins in one review, while one 2024 cryaa-promoter tool paper cites α-crystallin as ~22% of total lens protein. | (rossen2025zebrafishasa pages 3-4, rossen2025zebrafishasa pages 2-3, peng2024thegenerationand pages 1-2, zou2015aconservedrole media a1406ec4) |
| Tools/applications | Zebrafish-specific: the cryaa promoter is a robust lens-specific driver used for GFP, Cre, and mutant crystallin expression, enabling lens-targeted gene manipulation and cataract modeling. A 2024 transgenic line, zTg(cryaa:Cre-cryaa:EGFP), showed lens-specific Cre activity with ~98.4% recombination efficiency in one assay and no obvious developmental/lens transparency defect from transgene expression. | (peng2024thegenerationand pages 1-2, peng2024thegenerationand pages 6-6, peng2024thegenerationand pages 6-7, posner2019whydoesthe pages 1-2) |
Table: This table summarizes experimentally supported functional annotation for zebrafish cryaa/αA-crystallin (UniProt Q8UUZ6), separating zebrafish-specific findings from broader α-crystallin concepts. It highlights function, localization, developmental timing, phenotypes, quantitative observations, and practical uses of the cryaa promoter in zebrafish research.
References
(rossen2025zebrafishasa pages 3-4): Jennifer L. Rossen, Antionette L. Williams, and Brenda L. Bohnsack. Zebrafish as a model for crystallin-associated congenital cataracts in humans. Frontiers in Cell and Developmental Biology, Mar 2025. URL: https://doi.org/10.3389/fcell.2025.1552988, doi:10.3389/fcell.2025.1552988. This article has 5 citations.
(zou2015aconservedrole pages 1-2): Ping Zou, Shu-Yu Wu, Hanane A. Koteiche, Sanjay Mishra, Daniel S. Levic, Ela Knapik, Wenbiao Chen, and Hassane S. Mchaourab. A conserved role of αa-crystallin in the development of the zebrafish embryonic lens. Experimental eye research, 138:104-13, Sep 2015. URL: https://doi.org/10.1016/j.exer.2015.07.001, doi:10.1016/j.exer.2015.07.001. This article has 38 citations and is from a peer-reviewed journal.
(slingsby2013evolutionofcrystallins pages 1-2): Christine Slingsby, Graeme J. Wistow, and Alice R. Clark. Evolution of crystallins for a role in the vertebrate eye lens. Protein Science, 22:367-380, Apr 2013. URL: https://doi.org/10.1002/pro.2229, doi:10.1002/pro.2229. This article has 210 citations and is from a peer-reviewed journal.
(rossen2025zebrafishasa pages 2-3): Jennifer L. Rossen, Antionette L. Williams, and Brenda L. Bohnsack. Zebrafish as a model for crystallin-associated congenital cataracts in humans. Frontiers in Cell and Developmental Biology, Mar 2025. URL: https://doi.org/10.3389/fcell.2025.1552988, doi:10.3389/fcell.2025.1552988. This article has 5 citations.
(peng2024thegenerationand pages 2-2): Xuyan Peng, Xiaolin Jia, Guohui Shang, Mengjiao Xue, Mingjun Jiang, Dandan Chen, Fengyan Zhang, and Yanzhong Hu. The generation and characterization of a transgenic zebrafish line with lens-specific cre expression. Molecular Vision, 30:123-136, Jan 2024. URL: https://doi.org/10.63500/mv_v30_123, doi:10.63500/mv_v30_123. This article has 2 citations and is from a peer-reviewed journal.
(peng2024thegenerationand pages 1-2): Xuyan Peng, Xiaolin Jia, Guohui Shang, Mengjiao Xue, Mingjun Jiang, Dandan Chen, Fengyan Zhang, and Yanzhong Hu. The generation and characterization of a transgenic zebrafish line with lens-specific cre expression. Molecular Vision, 30:123-136, Jan 2024. URL: https://doi.org/10.63500/mv_v30_123, doi:10.63500/mv_v30_123. This article has 2 citations and is from a peer-reviewed journal.
(peng2024thegenerationand pages 6-7): Xuyan Peng, Xiaolin Jia, Guohui Shang, Mengjiao Xue, Mingjun Jiang, Dandan Chen, Fengyan Zhang, and Yanzhong Hu. The generation and characterization of a transgenic zebrafish line with lens-specific cre expression. Molecular Vision, 30:123-136, Jan 2024. URL: https://doi.org/10.63500/mv_v30_123, doi:10.63500/mv_v30_123. This article has 2 citations and is from a peer-reviewed journal.
(posner2019whydoesthe pages 1-2): Mason Posner, Matthew S. McDonald, Kelly L. Murray, and Andor J. Kiss. Why does the zebrafish cloche mutant develop lens cataract? PLOS ONE, 14:e0211399, Mar 2019. URL: https://doi.org/10.1371/journal.pone.0211399, doi:10.1371/journal.pone.0211399. This article has 11 citations and is from a peer-reviewed journal.
(goishi2006αacrystallinexpressionprevents pages 1-2): Katsutoshi Goishi, Akio Shimizu, Gabriel Najarro, Sumiko Watanabe, Rick Rogers, Leonard I. Zon, and Michael Klagsbrun. Αa-crystallin expression prevents γ-crystallin insolubility and cataract formation in the zebrafish cloche mutant lens. Development, 133:2585-2593, Jul 2006. URL: https://doi.org/10.1242/dev.02424, doi:10.1242/dev.02424. This article has 91 citations and is from a domain leading peer-reviewed journal.
(goishi2006αacrystallinexpressionprevents pages 6-7): Katsutoshi Goishi, Akio Shimizu, Gabriel Najarro, Sumiko Watanabe, Rick Rogers, Leonard I. Zon, and Michael Klagsbrun. Αa-crystallin expression prevents γ-crystallin insolubility and cataract formation in the zebrafish cloche mutant lens. Development, 133:2585-2593, Jul 2006. URL: https://doi.org/10.1242/dev.02424, doi:10.1242/dev.02424. This article has 91 citations and is from a domain leading peer-reviewed journal.
(wu2018transgeniczebrafishmodels pages 1-2): Shu-Yu Wu, Ping Zou, Sanjay Mishra, and Hassane S. Mchaourab. Transgenic zebrafish models reveal distinct molecular mechanisms for cataract-linked αa-crystallin mutants. PLOS ONE, 13:e0207540, Nov 2018. URL: https://doi.org/10.1371/journal.pone.0207540, doi:10.1371/journal.pone.0207540. This article has 21 citations and is from a peer-reviewed journal.
(zou2015aconservedrole pages 8-9): Ping Zou, Shu-Yu Wu, Hanane A. Koteiche, Sanjay Mishra, Daniel S. Levic, Ela Knapik, Wenbiao Chen, and Hassane S. Mchaourab. A conserved role of αa-crystallin in the development of the zebrafish embryonic lens. Experimental eye research, 138:104-13, Sep 2015. URL: https://doi.org/10.1016/j.exer.2015.07.001, doi:10.1016/j.exer.2015.07.001. This article has 38 citations and is from a peer-reviewed journal.
(zou2015aconservedrole media a1406ec4): Ping Zou, Shu-Yu Wu, Hanane A. Koteiche, Sanjay Mishra, Daniel S. Levic, Ela Knapik, Wenbiao Chen, and Hassane S. Mchaourab. A conserved role of αa-crystallin in the development of the zebrafish embryonic lens. Experimental eye research, 138:104-13, Sep 2015. URL: https://doi.org/10.1016/j.exer.2015.07.001, doi:10.1016/j.exer.2015.07.001. This article has 38 citations and is from a peer-reviewed journal.
(zou2015aconservedrole media d5eb8994): Ping Zou, Shu-Yu Wu, Hanane A. Koteiche, Sanjay Mishra, Daniel S. Levic, Ela Knapik, Wenbiao Chen, and Hassane S. Mchaourab. A conserved role of αa-crystallin in the development of the zebrafish embryonic lens. Experimental eye research, 138:104-13, Sep 2015. URL: https://doi.org/10.1016/j.exer.2015.07.001, doi:10.1016/j.exer.2015.07.001. This article has 38 citations and is from a peer-reviewed journal.
(zou2015aconservedrole media bc004cf0): Ping Zou, Shu-Yu Wu, Hanane A. Koteiche, Sanjay Mishra, Daniel S. Levic, Ela Knapik, Wenbiao Chen, and Hassane S. Mchaourab. A conserved role of αa-crystallin in the development of the zebrafish embryonic lens. Experimental eye research, 138:104-13, Sep 2015. URL: https://doi.org/10.1016/j.exer.2015.07.001, doi:10.1016/j.exer.2015.07.001. This article has 38 citations and is from a peer-reviewed journal.
(zou2015aconservedrole media a21c2392): Ping Zou, Shu-Yu Wu, Hanane A. Koteiche, Sanjay Mishra, Daniel S. Levic, Ela Knapik, Wenbiao Chen, and Hassane S. Mchaourab. A conserved role of αa-crystallin in the development of the zebrafish embryonic lens. Experimental eye research, 138:104-13, Sep 2015. URL: https://doi.org/10.1016/j.exer.2015.07.001, doi:10.1016/j.exer.2015.07.001. This article has 38 citations and is from a peer-reviewed journal.
(goishi2006αacrystallinexpressionprevents pages 4-5): Katsutoshi Goishi, Akio Shimizu, Gabriel Najarro, Sumiko Watanabe, Rick Rogers, Leonard I. Zon, and Michael Klagsbrun. Αa-crystallin expression prevents γ-crystallin insolubility and cataract formation in the zebrafish cloche mutant lens. Development, 133:2585-2593, Jul 2006. URL: https://doi.org/10.1242/dev.02424, doi:10.1242/dev.02424. This article has 91 citations and is from a domain leading peer-reviewed journal.
(wu2018transgeniczebrafishmodels pages 4-5): Shu-Yu Wu, Ping Zou, Sanjay Mishra, and Hassane S. Mchaourab. Transgenic zebrafish models reveal distinct molecular mechanisms for cataract-linked αa-crystallin mutants. PLOS ONE, 13:e0207540, Nov 2018. URL: https://doi.org/10.1371/journal.pone.0207540, doi:10.1371/journal.pone.0207540. This article has 21 citations and is from a peer-reviewed journal.
(wu2018transgeniczebrafishmodels pages 2-4): Shu-Yu Wu, Ping Zou, Sanjay Mishra, and Hassane S. Mchaourab. Transgenic zebrafish models reveal distinct molecular mechanisms for cataract-linked αa-crystallin mutants. PLOS ONE, 13:e0207540, Nov 2018. URL: https://doi.org/10.1371/journal.pone.0207540, doi:10.1371/journal.pone.0207540. This article has 21 citations and is from a peer-reviewed journal.
(yang2024reversiblecoldinducedlens pages 2-3): Hao Yang, Xiyuan Ping, Jiayue Zhou, Hailaiti Ailifeire, Jing Wu, Francisco M. Nadal-Nicolás, Kiyoharu J. Miyagishima, Jing Bao, Yuxin Huang, Yilei Cui, Xin Xing, Shiqiang Wang, Ke Yao, Wei Li, and Xingchao Shentu. Reversible cold-induced lens opacity in a hibernator reveals a molecular target for treating cataracts. The Journal of Clinical Investigation, Sep 2024. URL: https://doi.org/10.1172/jci169666, doi:10.1172/jci169666. This article has 15 citations.
(yang2024reversiblecoldinducedlens pages 8-10): Hao Yang, Xiyuan Ping, Jiayue Zhou, Hailaiti Ailifeire, Jing Wu, Francisco M. Nadal-Nicolás, Kiyoharu J. Miyagishima, Jing Bao, Yuxin Huang, Yilei Cui, Xin Xing, Shiqiang Wang, Ke Yao, Wei Li, and Xingchao Shentu. Reversible cold-induced lens opacity in a hibernator reveals a molecular target for treating cataracts. The Journal of Clinical Investigation, Sep 2024. URL: https://doi.org/10.1172/jci169666, doi:10.1172/jci169666. This article has 15 citations.
(yang2024reversiblecoldinducedlens pages 1-2): Hao Yang, Xiyuan Ping, Jiayue Zhou, Hailaiti Ailifeire, Jing Wu, Francisco M. Nadal-Nicolás, Kiyoharu J. Miyagishima, Jing Bao, Yuxin Huang, Yilei Cui, Xin Xing, Shiqiang Wang, Ke Yao, Wei Li, and Xingchao Shentu. Reversible cold-induced lens opacity in a hibernator reveals a molecular target for treating cataracts. The Journal of Clinical Investigation, Sep 2024. URL: https://doi.org/10.1172/jci169666, doi:10.1172/jci169666. This article has 15 citations.
(zou2015aconservedrole pages 9-9): Ping Zou, Shu-Yu Wu, Hanane A. Koteiche, Sanjay Mishra, Daniel S. Levic, Ela Knapik, Wenbiao Chen, and Hassane S. Mchaourab. A conserved role of αa-crystallin in the development of the zebrafish embryonic lens. Experimental eye research, 138:104-13, Sep 2015. URL: https://doi.org/10.1016/j.exer.2015.07.001, doi:10.1016/j.exer.2015.07.001. This article has 38 citations and is from a peer-reviewed journal.
(peng2024thegenerationand pages 7-8): Xuyan Peng, Xiaolin Jia, Guohui Shang, Mengjiao Xue, Mingjun Jiang, Dandan Chen, Fengyan Zhang, and Yanzhong Hu. The generation and characterization of a transgenic zebrafish line with lens-specific cre expression. Molecular Vision, 30:123-136, Jan 2024. URL: https://doi.org/10.63500/mv_v30_123, doi:10.63500/mv_v30_123. This article has 2 citations and is from a peer-reviewed journal.
(rossen2025zebrafishasa pages 4-5): Jennifer L. Rossen, Antionette L. Williams, and Brenda L. Bohnsack. Zebrafish as a model for crystallin-associated congenital cataracts in humans. Frontiers in Cell and Developmental Biology, Mar 2025. URL: https://doi.org/10.3389/fcell.2025.1552988, doi:10.3389/fcell.2025.1552988. This article has 5 citations.
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