MDH3 notes (P32419, YDL078C)

• Third isozyme, SKL PTS1, peroxisomal PMID:1447211
• Triple mutant lacks MDH activity PMID:1447211
• Beta-oxidation NADH reoxidation, not glyoxylate cycle PMID:7628449
• Matrix import as dimer PMID:8824293

Decisions

• Mitochondrion IBA and TCA cycle IBA/IEA from the MDH1 clade -> REMOVE (paralog relocated to peroxisomes).
• Cytosol RCAs (glyoxylate pathway, PWY3O-94) -> REMOVE.
• Glyoxylate cycle RCA -> non-core (contested: mild acetate defect vs van Roermund data).
• Module note: YeastCyc assigns MDH3 to gluconeogenesis I/TCA via the superpathway; evidence supports only a peroxisomal redox-shuttle role.