GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/sad-I/sad-I-uniprot.txt

UniProt entry Q88K05 for sad-I

Independent domain and functional-family classifications support the retained enzyme class; submitted names, EC labels, electronic GO lines and ARBA-only statements are not used as independent functional evidence.
For Sad-I, the NADP-named PTHR43217:SF2 subgroup is a substantive competing cofactor classification against the NAD-dependent FunFam. Neither is dismissed as experimental or privileged merely because it matches GOA.

"PANTHER; PTHR43217:SF2; SUCCINATE-SEMIALDEHYDE DEHYDROGENASE [NADP(+)]; 1."

PMID:23229889

Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium.

Salmonella YneI accepts both NAD and NADP; the abstract reports higher affinity for NAD. This establishes homolog chemistry, not the target protein kinetics.

"YneI can use both NAD(+) and NADP(+) as cofactors"

PMID:3355840

Properties and functions of two succinic-semialdehyde dehydrogenases from Pseudomonas putida.

P. putida contains separable NAD-preferring/4-hydroxyphenylacetate-induced and NADP-dependent/putrescine-induced SSADH activities, without a locus assignment.

"Under standard assay conditions this enzyme acts preferentially with NAD but reduces NADP at 9% of the rate observed for NAD."

file:PSEPK/sad-I/sad-I-deep-research-openscientist.md

OpenScientist deep-research report for sad-I

The provider proposes a Sad/YneI-like assignment for Q88K05 and a Lys157 cofactor-determinant mapping; these are research leads from its own alignment, not published locus-specific results.

file:modules/bacterial_4_hydroxybutyrate_oxidation-deep-research-openscientist.md

OpenScientist review of bacterial 4-hydroxybutyrate oxidation

The report treats Sad-family enzymes as NAD-preferring but potentially cofactor-promiscuous.

file:PSEPK/sad-I/sad-I-notes.md

Manual evidence notes for sad-I

Append-only manual notes distinguish target evidence, homolog inference and unresolved pathway assignments.