Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Bacterial Interactomes: Interacting Protein Partners Share Similar Function and Are Validated in Independent Assays More Frequently Than Previously Reported.
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High-confidence AP-MS survey identifies 459 PPIs in D. vulgaris
"We have identified 459 high confidence PPIs from D. vulgaris"
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Documents RpoC-RpoB interaction via AP-MS
"Compared with the nine published interactomes, our two networks are smaller, are much less highly connected, and have significantly lower false discovery rates"
Quantitative Tagless Copurification: A Method to Validate and Identify Protein-Protein Interactions.
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Validates D. vulgaris protein interactions through orthogonal chromatography
"5273 fractions from a four-step fractionation of a D. vulgaris protein extract were assayed, resulting in the detection of 1242 proteins"
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RpoC-RpoB copurification confirmed through tagless copurification
"Protein partners from our D. vulgaris and E. coli AP-MS interactomes copurify as frequently as pairs belonging to three benchmark data sets of well-characterized PPIs"
Deep research summary for rpoC (Q727C6) in D. vulgaris
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DVU_2929 maps to rpoC with peptide-level proteomic detection
"DVU_2929 encodes RpoC (RNAP beta') in DvH, supported by direct locus mapping, proteomic detection, and PPI evidence"
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Documents essential role of trigger loop in initiation, elongation, and termination
"Trigger loop functions in all three phases of the transcription cycle"
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Describes clamp/switch dynamics essential for DNA loading
"Recent (2023-2024) structural work refines initiation models centered on beta' clamp dynamics and sigma loading"