Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Interactions between Kar2p and its nucleotide exchange factors Sil1p and Lhs1p are mechanistically distinct.
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The ER Hsp70 Kar2p (BiP) is regulated by two nucleotide-exchange factors, Sil1p and Lhs1p; NEFs promote ADP release to drive the next round of ATP binding in the chaperone cycle.
"Two NEFs have been identified for Kar2p in the ER lumen of Saccharomyces cerevisiae, namely Lhs1p and Sil1p"
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Sil1p binds the IIB subdomain of the Kar2p ATPase domain to stimulate nucleotide exchange, mechanistically distinct from Lhs1p.
"These studies identified a role for the IIB domain of Kar2p in Sil1p binding and Sil1p-stimulated nucleotide exchange"
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Single deletions of SIL1 or LHS1 are viable but the double deletion is lethal, showing BiP nucleotide exchange is essential and the two NEFs are partly redundant.
"Individual deletions of LHS1 (Δlhs1) and SIL1 (Δsil1) in yeast cells are viable (13, 14), but the double deletion (Δlhs1Δsil1) is lethal suggesting that nucleotide exchange is essential for Kar2p activity and cell viability"
Broad functional profiling of fission yeast proteins using phenomics and machine learning.
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Genome-wide phenomics/machine-learning profiling of S. pombe deletion mutants; the study explicitly targets poorly characterized proteins, and sil1 phenotypes in PomBase derive from this class of broad screen rather than a dedicated study.
"Many proteins remain poorly characterized even in well-studied organisms"
A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
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Systematic mating-morphology deletion screen in S. pombe; the cell-fusion phenotype recorded for sil1 in PomBase (incomplete cell wall disassembly at the fusion site) originates from this genome-wide screen.
"morphological abnormalities of the mating process in fission yeast"