Increased susceptibility to cortical spreading depression in the mouse model of familial hemiplegic migraine type 2.
Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Transport and pharmacological properties of nine different human Na, K-ATPase isozymes.
-
Human alpha/beta isozymes expressed in Xenopus oocytes have functional pump currents; selected original Methods and Figure 6 additionally distinguish ouabain-sensitive currents from ligand-binding measurements.
"all
human isozymes are functional"
The Na(+)-K(+)-ATPase alpha2 gene and trainability of cardiorespiratory endurance: the HERITAGE family study.
Haploinsufficiency of ATP1A2 encoding the Na+/K+ pump alpha2 subunit associated with familial hemiplegic migraine type 2.
New molecular determinants controlling the accessibility of ouabain to its binding site in human Na,K-ATPase alpha isoforms.
-
Human alpha1/alpha2 constructs were compared for ouabain-binding kinetics in microsomal preparations; a cellular response is not established by this assay alone.
"measured their ouabain
binding kinetics."
Purification of the human alpha2 Isoform of Na,K-ATPase expressed in Pichia pastoris. Stabilization by lipids and FXYD1.
-
Human alpha2 paired with porcine beta1 was purified from Pichia in an active complex; preparation and partner species qualify the biochemical interpretation.
"Detergent-soluble
alpha2beta1 and alpha1beta1 complexes have been purified in a stable and
functional state."
Diverse functional consequences of mutations in the Na+/K+-ATPase alpha2-subunit causing familial hemiplegic migraine type 2.
-
Human alpha2 variants affect pump transport, membrane expression and ion handling in Xenopus oocytes; the reference construct carries Q116R/N127D for ouabain resistance.
"pronounced
and markedly diverse functional alterations in all ATP1A2 mutants."
Impaired plasma membrane targeting or protein stability by certain ATP1A2 mutations identified in sporadic or familial hemiplegic migraine.
Dysfunction of ouabain-induced cardiac contractility in mice with heart-specific ablation of Na,K-ATPase beta1-subunit.
-
Mouse cardiac beta1 deletion alters contractile physiology while alpha2 protein abundance remains unchanged; the inspected work does not establish intercellular electrical current transfer.
"The level of Na,K-α2 protein however remained unchanged."
Isoform specificity of cardiac glycosides binding to human Na+,K+-ATPase alpha1beta1, alpha2beta1 and alpha3beta1.
Hyperpolarization-activated inward leakage currents caused by deletion or mutation of carboxy-terminal tyrosines of the Na+/K+-ATPase {alpha} subunit.
-
A human alpha2 reference and carboxy-terminal mutants show conditional inward currents; proton leak in Na/K-free acidic conditions is not the physiological pump core.
"H(+)
leak currents of the wild-type Na(+)/K(+)-ATPase"
Coordinated regulation of cardiac Na(+)/Ca (2+) exchanger and Na (+)-K (+)-ATPase by phospholemman (FXYD1).
Na⁺ transport in the normal and failing heart - remember the balance.
-
The review distinguishes pump-mediated repolarization from the depolarizing action-potential phase and discusses cardiac sodium/calcium coupling.
"By extruding one net positive charge, NKA participates in membrane repolarization"
Familial hemiplegic migraine mutations affect Na,K-ATPase domain interactions.
Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Blood-Brain Barrier: From Physiology to Disease and Back.
ATP1A:ATP1B:FXYD exchanges 3Na+ for 2K+
ATP1A:ATP1B:FXYD binds cardiac glycosides
Degeneration of the amygdala/piriform cortex and enhanced fear/anxiety behaviors in sodium pump alpha2 subunit (Atp1a2)-deficient mice.
Malfunction of respiratory-related neuronal activity in Na+, K+-ATPase alpha2 subunit-deficient mice is attributable to abnormal Cl- homeostasis in brainstem neurons.