CG14662 (Q9VN74): evidence and ProtNLM claim review

CG14662 is a 550-residue leucine-rich-repeat protein with two predicted hydrophobic membrane-spanning segments. It is a plausible membrane interaction protein, while ligand recognition, membrane topology, and physiological function remain unresolved.

Exact input: Q9VN74, 550 residues. The accession was fetched explicitly with the gene-directory alias; no canonical-sequence substitution is made.

Raw emitted predictions: CG14662-predictions-source.json. Source features: CG14662-uniprot.txt, with an exact extraction in CG14662-sequence-evidence.json.

Sequence and domain evidence

These are sequence/domain observations or explicitly named feature predictions, not measurements of biological function. ARBA assertions and ProtNLM-derived UniProt names are not counted as validation.

DR   InterPro; IPR032675; LRR_dom_sf.
FT   TRANSMEM        29..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        421..447
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"

ProtNLM claims

The snapshot emits names and location/keyword statements, with no GO or EC prediction for this target. Each actual statement is assessed below; no GO term has been substituted for it. Categories follow the function-prediction rubric, with nonspecific “uncharacterized” names marked UNC because they contain no testable function. CNN records an independently supported existing annotation; it does not assert a particular training-set composition.

Kind	Verbatim emitted statement	Assessment	Evidence and limitation
Name	Uncharacterized protein	UNC	Uncharacterized protein supplies no testable activity claim. The LRR fold suggests an interaction scaffold without specifying ligands.
Location	Membrane (SL-0162)	COR	Two independently called Phobius transmembrane segments support the broad membrane hypothesis. The result remains a sequence-based localization inference; it does not identify topology or organelle residence.

Literature evidence

No target-specific primary finding is used to establish a molecular activity here. The current assessment is bounded by exact-record architecture and the explicitly identified curated inferences.

Annotation decisions

GO:0003674 molecular_function (ND): ACCEPT. A leucine-rich-repeat scaffold can mediate many interactions and does not establish a specific receptor or ligand-binding activity.
GO:0005575 cellular_component (ND): UNDECIDED. Two Phobius hydrophobic segments at residues 29-50 and 421-447 suggest membrane insertion. The source GOA unknown-component placeholder and sequence-level inference do not resolve localization or topology experimentally.
GO:0008150 biological_process (ND): ACCEPT. Neither an LRR domain nor membrane-spanning architecture identifies the pathway in which this protein acts.

Research provenance

Genuine external literature research is requested through the repository Falcon wrapper, with perplexity-lite configured as fallback. Provider output is retained separately as CG14662-deep-research-<provider>.md; its source leads are checked against the underlying publications and exact sequence record.