Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Pregnenolone stabilizes microtubules and promotes zebrafish embryonic cell movement.
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Primary zebrafish morphant and rescue study supporting pregnenolone production and developmental effects. Later stable-knockout results in PMID36332486 limit unconditional necessity claims; no direct microtubule-binding function is attributed to CYP11A.
"pregnenolone, which is produced from cholesterol by the steroidogenic enzyme Cyp11a1 (cholesterol side-chain cleavage enzyme, P450scc)"
Redefining the initiation and maintenance of zebrafish interrenal steroidogenesis by characterizing the key enzyme cyp11a2.
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Primary paralog comparison: cyp11a1 is current cyp11a1.1 and cyp11a2 is current cyp11a1.2. Abstract explicitly separates early-development/gonadal from sustained interrenal expression and describes morpholino/pregnenolone rescue evidence. Do not generalize morpholino necessity to all genetic backgrounds.
"cyp11a2 is expressed in adult steroidogenic tissues, such as the interrenal, gonads, and brain. In contrast, cyp11a1 was mainly restricted to the gonads."
Pregnenolone activates CLIP-170 to promote microtubule growth and cell migration.
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Primary mechanistic work identifies CLIP-170 as the pregnenolone-binding microtubule effector. The cached abstract does not detail every cyp11a1 genetic interaction; independent PMID16437115 supports the product-mediated regulation claim.
"P5 activates CLIP-170 to promote microtubule polymerization and cell migration."
Fine selection of up-regulated genes during ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish.
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Full text read. Ovarian steroid-treatment expression profiling names NM_152953 in tables/primers, independently mapping the response to cyp11a1.1. Expression evidence is not treated as a novel physiological enzyme activity.
"cyp11a1NM_152953Danio rerio cytochrome P450"
Pathway-specific protein domains are predictive for human diseases.
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Full text does contain zebrafish heart/vascular morpholino experiments despite its broad title. The gene is rendered cypna1; exact assayed-construct mapping to the curated cyp11a1.1 record remains unresolved, so heart asymmetry is UNDECIDED rather than rejected as misattribution.
"the tram1, cypna1, and slc22a2 groups"
Evaluation of two gene ablation methods for the analysis of pregnenolone function in zebrafish embryonic development.
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Primary comparison explicitly reports normal stable cyp11a1 knockout lines despite morphant cell-migration defects. It qualifies necessity claims without disproving the enzyme chemistry or the entire pregnenolone developmental mechanism.
"Both homozygous cyp11a1 knockout lines appeared normal."
UniProtKB entry for cyp11a1.1 (Q7SYJ6)
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ZFIN/RefSeq cross-references map the current tandem-duplicate symbol to the historical literature gene. RuleBase RU364077 supplies catalytic and localization inferences; PIRSR supplies conserved cofactor support. These are not native-protein localization or metal-occupancy assays.
"A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone"