Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Matsuyama et al. generated a proteome-scale S. pombe YFP localization dataset that PomBase used for erg2 ER curation.
"we determined the localization of 4,431 proteins"
Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe.
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Iwaki et al. tested S. pombe late ergosterol biosynthetic genes, including erg2, and showed that the deletion series could not synthesize ergosterol.
"Disruption mutants of these genes were unable to synthesize ergosterol"
PANTHER PTHR10868 PAINT annotations
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PTN001280219 is the fungal PAINT node that carries the C-8 sterol isomerase and ergosterol-biosynthesis assertions for PTHR10868.
"PTHR10868 PTN001280219 GO:0000247 F IBD false CGD:CAL0000177093|SGD:S000004815 taxon:451864 20251127"
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PTN001328958 is the broader eukaryotic PAINT node that propagates only ER localization, not the animal Sigma-receptor-specific terms, to fungal ERG2 proteins.
"PTHR10868 PTN001328958 GO:0005783 C IBD false MGI:MGI:1195268|RGD:68364|SGD:S000004815|UniProtKB:Q99720 taxon:2759 20260528"
UniProtKB record for S. pombe erg2
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S. pombe erg2 is annotated as the ER-membrane C-8 sterol isomerase that catalyzes the fecosterol-to-episterol reaction during ergosterol biosynthesis.
"Reaction=fecosterol = episterol; Xref=Rhea:RHEA:33435"
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UniProt places S. pombe Erg2 at the endoplasmic reticulum membrane.
"SUBCELLULAR LOCATION: Endoplasmic reticulum membrane"
Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast.
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A 2025 fission-yeast lipidomics study found erg2 deletion among ergosterol-pathway backgrounds that resist pentadecanoic-acid lipotoxicity and giant ER sheet formation.
"C15:0 exhibited weaker or no activity against mutants lacking ergosterol biosynthetic genes such as erg2, erg31 and erg32, and erg5"