Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
DSCAM: a novel member of the immunoglobulin superfamily maps in a Down syndrome region and is involved in the development of the nervous system.
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DSCAM is a novel IgSF cell adhesion molecule with 10 Ig-C2 domains and 6 FNIII domains, existing as two isoforms (Long with transmembrane domain, Short without), expressed primarily in brain during neuronal differentiation.
"The sequence of cDNAs indicates alternative splicing and predicts two protein isoforms, both containing 10 Ig-C2 domains...with or without the following transmembrane and intracellular domains"
Down syndrome cell adhesion molecule DSCAM mediates homophilic intercellular adhesion.
DSCAM is a netrin receptor that collaborates with DCC in mediating turning responses to netrin-1.
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DSCAM is expressed on spinal commissural axons, binds netrin-1, and is necessary for commissural axons to grow toward and across the midline. DSCAM and DCC can each mediate a turning response to netrin-1 independently.
"the Down's syndrome Cell Adhesion Molecule (DSCAM) ...is expressed on spinal commissural axons, binds netrin-1, and is necessary for commissural axons to grow toward and across the midline. DSCAM and DCC can each mediate a turning response of these neurons to netrin-1"
DSCAM functions as a netrin receptor in commissural axon pathfinding.
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DSCAM interacts with Netrin-1 and independently of DCC mediates netrin signaling by activating phosphorylation of Fyn and Pak1. Knockdown causes commissural axon projection and pathfinding defects.
"DSCAM by itself, in the absence of DCC, is capable of mediating netrin signaling in activating phosphorylation of Fyn and Pak1"
Down syndrome cell adhesion molecule (DSCAM) associates with uncoordinated-5C (UNC5C) in netrin-1-mediated growth cone collapse.
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DSCAM interacts with UNC5C in a netrin-1-stimulated manner and coordinates with UNC5C in netrin-1-mediated growth cone collapse (repulsion). Netrin-1 increases tyrosine phosphorylation of DSCAM, UNC5C, FAK, Fyn, and PAK1 and promotes complex formation.
"DSCAM interacts with UNC5C and this interaction is stimulated by netrin-1 in primary cortical neurons and postnatal cerebellar granule cells"
A reference map of the human binary protein interactome.
A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions.
Chr21 protein-protein interactions: enrichment in proteins involved in intellectual disability, autism, and late-onset Alzheimer's disease.
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DSCAM intracellular domain binds DLG2 (scaffold at postsynaptic density) and DYRK1A. Some HSA21 proteins including DSCAM were located at the dendritic spine postsynaptic density.
"an intracellular domain of DSCAM bound either DLGs, which are multimeric scaffolds comprising receptors, ion channels and associated signaling proteins, or DYRK1A"
DSCAM/DSCAML1 homodimerization