"PEX19 binds a broad spectrum of PMPs, displays saturable PMP binding, and interacts with regions of PMPs required for their targeting to peroxisomes."

"At steady state, PEX19 is bimodally distributed between the cytoplasm and peroxisome, with most of the protein in the cytoplasm."

"the loss of PEX19 results in degradation of PMPs and/or mislocalization of PMPs to the mitochondrion."

"we identified the peroxin PEX19p as a novel interactor of ALDP, ALDRP, and PMP70."

"Our data provide evidence that PEX19p is a cytosolic acceptor protein for the peroxisomal ABC transporters ALDP, PMP70, and ALDRP and might be involved in the intracellular sorting and trafficking of these proteins to the peroxisomal membrane."

"the metabolite transporter PMP34, an integral PMP, contains at least two nonoverlapping sets of targeting information, either of which is sufficient for insertion into the peroxisome membrane."

"we demonstrate that PEX19, a factor required for peroxisomal membrane biogenesis, interacts with the two minimal targeting regions of PMP34."

"peroxin 19 (PEX19) interactions are not required for targeting human PMP70 to peroxisomes."

"PEX19 does not specifically bind to the targeting elements of human PMP70."

"Both of these peroxisomal targeting regions interact with PEX19, a factor required for peroxisome membrane synthesis."

"farnesylation, and not the CAAX motif, of Pex19p strongly enhances its affinity for Pex13p"

"the CAAXmotif, and not farnesylation, of Pex19p strongly enhances its affinity for Pex11pbeta"

"homopolymerisation of Pex14p involves a domain comprising amino acid residues 147-278 of this peroxin."

"We show here that PEX19 has all of the properties one would expect for a bifunctional PMP chaperone/import receptor."

"PEX19 binds and stabilizes newly synthesized PMPs in the cytosol, binds to multiple PMP targeting signals (mPTSs), interacts with the hydrophobic domains of PMP targeting signals, and is essential for PMP targeting and import."

"We also demonstrate the existence of two PMP import mechanisms and two classes of mPTSs: class 1 mPTSs, which are bound by PEX19 and imported in a PEX19-dependent manner, and class 2 mPTSs, which are not bound by PEX19 and mediate protein import independently of PEX19."

"Pex19p translocates the membrane peroxins from the cytosol to peroxisomes in an ATP- and Pex3p-dependent manner and then shuttles back to the cytosol."

"Cell-free synthesized, 35S-labeled Pex19p was targeted to subcellular fractions containing peroxisomes from Chinese hamster ovary-K1 cells as well as peroxisomes isolated from rat liver in an ATP-dependent manner."

"Peroxisome-associated and partly Na2CO3-resistant 35S-Pex19p was released to the cytosolic fraction upon incubation in the absence of ATP, whereas 35S-Pex16p and 35S-Pex26p remained in the membranes."

"When PMP70 was translated in the presence of purified Pex19p, a large part of PMP70 existed as soluble form and was co-immunoprecipitated with Pex19p."

"in the absence of Pex19p, PMP70 formed aggregates during translation."

"These results suggest that Pex19p binds to PMP70 co-translationally and keeps PMP70 as a proper conformation for the localization to peroxisome."

"Its C-terminal-targeting signal contains two binding sites for PEX19, the import receptor for several peroxisomal membrane proteins."

"we show that PEX19 is essential for PEX26 import."

"We conclude that C-terminal PEX19-binding sites mark tail-anchored proteins for delivery to peroxisomes."

"Gel filtration chromatography analyses and intrinsic tryptophan fluorescence titrations revealed that a one-to-one complex is formed between monomeric Pex3p and monomeric Pex19p."

"the wild-type and the W104A and W104F mutants showed K(D) values of 3.4 nm, 1080 nm, and 66.2 nm, respectively."

"the indole ring of Trp-104 directly interacts with Pex19p to facilitate the specific peroxisomal translocation of the Pex19p-PMP complexes."

"Here we demonstrate for the first time that peroxisomal targeting of hFis1 depends on Pex19p, a peroxisomal membrane protein import factor."

"Silencing of Pex19p by small interference RNA reduced the targeting of hFis1 to peroxisomes, but not to mitochondria."

"Our findings indicate that targeting of hFis1 to peroxisomes and mitochondria are independent events and support a direct, Pex19p-dependent targeting of peroxisomal tail-anchored proteins."

"We show here that Pex19p plays an essential role as the chaperone for full-length Pex3p in the cytosol."

"Knockdown of Pex19p inhibits peroxisomal targeting of newly synthesized full-length Pex3p"

"Pex16p is the membrane receptor for Pex3p"

"Pex5 and Pex19 ligand helices bind competitively to the same surface in Pex14(N) albeit with opposite directionality."

"The molecular recognition involves conserved aromatic side chains in the Pex5 WxxxF/Y motif and a newly identified F/YFxxxF sequence in Pex19."

"a recombinant protein comprising the cytosolic domain of PEX3 can be purified in a soluble and monomeric form in the absence of detergents or other solubilizing agents."

"we tested this recombinant protein in lipid-binding assays and found that it interacts strongly with liposomes inducing their flocculation or even partial solubilization."

"The crystal structure of the folded C-terminal part of the receptor reveals a globular domain that displays a bundle of three long helices in an antiparallel arrangement."

"the structured alpha-helical domain binds PMP-targeting signal (mPTS) sequences with about 10 muM affinity."

"we present the three-dimensional structure of the complex between a cytosolic domain of Pex3p and the binding-region peptide of Pex19p."

"A 16-residue region of the Pex19p peptide forms an alpha-helix and makes a contact with Pex3p; this helix is disordered in the unbound state."

"The Pex19p peptide contains a characteristic motif, consisting of the leucine triad (Leu18, Leu21, Leu22), and Phe29, which are critical for the Pex3p binding and peroxisome biogenesis."

"Using mass spectrometric analysis, almost all known human peroxins involved in protein import were identified as constituents of the PEX14 complexes."

"For the HsPXF protein, a carboxyterminal farnesylation at cysteine residues was demonstrated."

"Through the use of HsPXF-specific antibodies, the protein was shown to be attached to the outer surface of peroxisomes."

"we detected four splice variants originating either from exon skipping or from alternative splicing events."

"We isolated a 33-kDa protein, Pex19p/HK33/HsPXF, as a p19ARF-binding protein in a yeast two-hybrid screen."

"Pex19p interacts with p19ARF in the cell cytoplasm and excludes p19ARF from the nucleus, leading to a concurrent inactivation of p53 function."

"Down-regulation of Pex19p by its antisense expression resulted in increased levels of p19ARF, increased p53 function, and a p53/p21WAF1-mediated senescence-like cell cycle arrest."