Gene Ontology annotation through association of InterPro records with GO terms

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/aceA/aceA-deep-research-falcon.md

Deep research report (Falcon/Edison) for aceA (PP_4116), Pseudomonas putida KT2440

Synthesizes literature establishing aceA/PP_4116 as isocitrate lyase of the glyoxylate shunt, required for growth on acetyl-CoA-yielding substrates (fatty acids, octane) in KT2440.

PMID:30200852

The Glyoxylate Shunt, 60 Years On

Isocitrate lyase (AceA) cleaves isocitrate into succinate and glyoxylate, the committed step of the glyoxylate shunt, which is a branch of the TCA cycle that bypasses the two oxidative decarboxylation steps to conserve carbon.

PMID:35651318

Providing octane degradation capability to Pseudomonas putida KT2440 through the horizontal acquisition of oct genes located on an integrative and conjugative element

aceA (PP_4116, isocitrate lyase) is induced during alkane assimilation and an aceA knockout fails to grow on octane/octanoic acid, demonstrating its requirement for routing acetyl-CoA through the glyoxylate shunt in KT2440.

PMID:24951791

The functional structure of central carbon metabolism in Pseudomonas putida KT2440

13C flux analysis shows glyoxylate-cycle flux is carbon-source dependent in KT2440 (no detectable flux on glucose; ~4% of isocitrate flux diverted through the glyoxylate cycle on fructose).