Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Transcriptional regulation of the human asparagine synthetase gene by carbohydrate availability.
Efficient expression, purification, and characterization of C-terminally tagged, recombinant human asparagine synthetase.
Enhanced expression of asparagine synthetase under glucose-deprived conditions protects pancreatic cancer cells from apoptosis induced by glucose deprivation and cisplatin.
A proteome-scale map of the human interactome network.
Expression of human asparagine synthetase in Escherichia coli.
Organization and expression of the cell cycle gene, ts11, that encodes asparagine synthetase.
The N-terminal cysteine of human asparagine synthetase is essential for glutamine-dependent activity.
Isolation of human cDNAs for asparagine synthetase and expression in Jensen rat sarcoma cells.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
aspartate + glutamine + ATP <=> asparagine + glutamate + AMP + pyrophosphate [ASNS]
ASNS manual primary-reading and source-boundary notes
Immutable fetched human ASNS UniProt record
3D variability analysis reveals a hidden conformational change controlling ammonia transport in human asparagine synthetase.
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Unmodified human ASNS forms a head-to-head dimer with glutaminase and synthetase domains.
"First, two monomers of unmodified human ASNS in the native state form a head-to-head dimer as a result of intermolecular interactions involving residues 31–34 in the adjacent N-terminal domains."
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Human WT and tunnel variants were compared under glutamine-fed and free-ammonia-fed in-vitro conditions using a PPi assay.
"The rate of inorganic pyrophosphate (PPi) production catalyzed by recombinant, WT human ASNS and two ammonia tunnel variants was determined in a continuous assay format using the EnzChek™ Pyrophosphate Assay Kit (Molecular Probes, Eugene, OR)."