Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
PTHR11635 PAINT annotations
Integrative, multifunctional plasmids for hypha-specific or constitutive expression of green fluorescent protein in Candida albicans.
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Constitutive BCY1 overexpression inhibited C. albicans germ tube formation and filamentation.
"Strains overexpressing BCY1 from the ENO1 promoter were inhibited in germ tube formation and filamentation in both liquid and solid media"
Candida albicans lacking the gene encoding the regulatory subunit of protein kinase A displays a defect in hyphal formation and an altered localization of the catalytic subunit.
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In a bcy1 tpk2 double mutant, PKA activity was constitutive and cAMP-independent.
"protein kinase A activity in the bcy1 tpk2 mutant was independent of the presence of cAMP"
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Bcy1 interacts with the Tpk1 catalytic subunit in C. albicans cells.
"Coimmunoprecipitation of Tpk1-GFP with Bcy1p in ASM1 and ASM2 strongly suggests interaction of the two proteins inside the cell."
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The paper did not directly image Bcy1p; its Bcy1-GFP nuclear claim was a preliminary author statement.
"Although Bcy1p was not visualized directly in our experiments"
Expression levels and subcellular localization of Bcy1p in Candida albicans mutant strains devoid of one BCY1 allele results in a defective morphogenetic behavior.
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Bcy1p-GFP co-eluted with phosphotransferase activity, suggesting that the GFP tag did not prevent interaction with catalytic subunits.
"was not impaired by the GFP tag"
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The authors interpreted Bcy1p-GFP localization as supporting nuclear localization of the regulatory subunit.
"nuclear localization of the regulatory subunit"
Catalytic isoforms Tpk1 and Tpk2 of Candida albicans PKA have non-redundant roles in stress response and glycogen storage.
The Regulatory Subunit of Protein Kinase A (Bcy1) in Candida albicans Plays Critical Roles in Filamentation and White-Opaque Switching but Is Not Essential for Cell Growth.
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A bcy1/bcy1 null mutant could be constructed in a laboratory wild-type strain of C. albicans.
"we successfully deleted both alleles of BCY1 and generated a bcy1/bcy1 null mutant in a laboratory wild type strain of C. albicans"
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BCY1 deletion caused multiple morphologies and hyperfilamentation in certain media.
"Deletion of BCY1 in C. albicans leads to multiple cellular morphologies and hyperfilamentation in certain media"
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Bcy1 regulates carbon-source utilization and white-opaque switching in C. albicans.
"We further show that Bcy1 plays an important role in the regulation of carbon source utilization and in white-opaque switching"
Exploring the differential localization of protein kinase A isoforms in Candida albicans.
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The Bcy1-GFP allele used for subcellular imaging was functional in growth and morphology assays.
"Bcy1-GFP was able to maintain growth comparable with cells expressing wild-type Bcy1"
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The Candida PKA holoenzyme is mainly cytosolic and Bcy1 is largely excluded from the nucleus under both glucose and glycerol conditions.
"Bcy1 is largely excluded from the nucleus under both conditions"
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Bcy1 and Tpk2 localize to the vacuolar membrane when cells grow on glycerol.
"Tpk2 and Bcy1 localize to the vacuolar membrane when C. albicans utilize an alternative carbon source such as glycerol"
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Bcy1-GFP associates with P-bodies and stress granules during heat stress.
"Strong association was seen between Dhh1-mSc and mNG-Tpk1, mNG-Tpk2, and Bcy1-GFP"
Ras1-Independent High Iron-Mediated Hyphal Formation in Candida albicans.
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BCY1 expression falls under high iron in ras1-null cells, and Bcy1 overexpression blocks iron-induced hyphal formation.
"Supporting this, overexpression of Bcy1 blocked iron-induced hyphal formation"
Isolation and characterisation of cAMP-dependent protein kinase from Candida albicans. Purification of the regulatory and catalytic subunits.
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Native C. albicans PKA was purified as a cAMP-responsive tetramer containing two regulatory subunits and two catalytic subunits.
"two regulatory (R) subunits of 64 kDa"
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cAMP activates native C. albicans PKA.
"150 nM with a Hill coefficient of 1.6"
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8-azido-cAMP photoaffinity labeling indicated that C. albicans has a single native PKA regulatory-subunit type.
"Photoaffinity labeling with 8-azido-[32P]cAMP of crude extracts from yeast and mycelial cells strongly suggests that only one type of R subunit is present in the fungus"