Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-specific components in fission yeast.
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Spo2 bridges the Spo15 and Spo13 components of the meiotic spindle pole body.
"Spo2 physically
associated with both Spo15 and Spo13, but Spo13 and Spo15 did not interact
directly."
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Recruitment is hierarchical, Spo15 then Spo2 then Spo13.
"Localization of Spo13 to the SPB
was dependent on Spo15 and Spo2; that of Spo2 depended only on Spo15"
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spo2 is dispensable for meiotic divisions but essential for sporulation, and the deletion blocks SPB modification and forespore membrane initiation.
"The
respective deletion mutants are viable, but defective in SPB modification and in
the onset of FSM formation."
UniProtKB C6Y4C2: curated sequence, features and source attribution
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UniProt curators (ECO:0000269, PMID:18367542) state that Spo2 helps localize Spo13 to the outer SPB surface.
"localization of spo13 to the outer surface of the SPB"
Spo2 (C6Y4C2) vs Vps1302 (O42926) sequence placement and signature analysis
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Spo2 equals Vps1302 residues 2999-3131 and no VPS13 domain signature overlaps that segment.
"None of the VPS13 domain signatures (N-terminal chorein region, middle region, adaptor-binding domain, C-terminal region, PTHR16166 span) overlaps the Spo2 segment."