Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Deep research report (falcon/Edison) for wzm (Q9HTB8) in P. aeruginosa PAO1
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Wzm is the transmembrane-domain (permease) subunit of the Wzm-Wzt ABC transporter that exports the UndPP-linked common polysaccharide antigen (CPA; A-band, a D-rhamnan homopolymer) from the cytoplasmic to the periplasmic face of the inner membrane; loss of wzm blocks export but not polymer synthesis.
"This D-rhamnan polymer is assembled on the cytoplasmic face of the inner membrane on a lipid carrier (UndPP), and Wzm–Wzt transports the completed polymer across the membrane to the periplasmic face"
Determination of the quaternary structure of a bacterial ATP-binding cassette (ABC) transporter in living cells.
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The A-band LPS polysaccharide is synthesized in the cytoplasm and translocated to the periplasm by an ABC transporter composed of the transmembrane protein Wzm and the cytoplasmic nucleotide-binding protein Wzt.
"The polysaccharides in A-band LPSs are synthesized in the cytoplasm and translocated into the periplasm via an ATP-binding cassette (ABC) transporter consisting of a transmembrane protein, Wzm, and a cytoplasmic nucleotide-binding protein, Wzt."
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Wzm forms a homotetramer that assembles with a Wzt tetramer into a hetero-octameric transporter complex.
"Wzm forms a square-shaped homo-tetramer both in the presence and absence of Wzt"
Identification and functional characterization of an ABC transport system involved in polysaccharide export of A-band lipopolysaccharide in Pseudomonas aeruginosa.
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Wzm is an integral inner-membrane protein with six potential membrane-spanning domains and, with the ATP-binding protein Wzt, forms an ABC transport system.
"Wzm is an integral membrane protein with six potential membrane-spanning domains, while Wzt is an ATP-binding protein containing a highly conserved ATP-binding motif."
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Chromosomal wzm mutants still synthesize A-band polysaccharide but fail to export it to the cell surface, so the polymer accumulates in the cytoplasm; B-band LPS synthesis is unaffected.
"the wzm and wzt mutants were able to synthesize A-band polysaccharide, although transport of the polymer to the cell surface was inhibited. The inability of the polymer to cross the inner membrane resulted in the accumulation of cytoplasmic A-band polysaccharide."