"Complementation studies in the yeast knockout demonstrate that the human homolog encodes alpha-aminoadipate dehydrogenase phosphopantetheinyl transferase activity."

"The human enzyme, which is expressed mainly in the cytosolic compartment in a wide range of tissues, is a 329-residue, monomeric protein."

"The enzyme is capable of transferring the 4'-phosphopantetheine moiety of coenzyme A to a conserved serine residue in both the acyl carrier protein domain of the human cytosolic multifunctional fatty acid synthase and the acyl carrier protein associated independently with human mitochondria."

"Using a stringent, high-throughput yeast two-hybrid system, we tested pairwise interactions among the products of approximately 8,100 currently available Gateway-cloned open reading frames and detected approximately 2,800 interactions."

"We determined the crystal structure of the human phosphopantetheinyl transferase, a eukaryotic phosphopantetheinyl transferase characterized, complexed with CoA and Mg(2+), and in ternary complex with CoA and ACP. The involvement of key residues in ligand binding and catalysis was confirmed by mutagenesis and kinetic analysis."

"Besides the structural data observed in the complexes, the role of Glu181 and Lys185 as key acid/base catalysts is strongly supported by the observed significant loss of activity for the Glu181Gln, Glu181Ala, and Lys185Ala mutant proteins."

"Here, we used LC-MS/MS to profile the proteome of human urinary exosomes. Overall, the analysis identified 1132 proteins unambiguously, including 177 that are represented on the Online Mendelian Inheritance in Man database of disease-related genes, suggesting that exosome analysis is a potential approach to discover urinary biomarkers."

"We identified 774 candidate interacting proteins associated with 75 Dubs."

"In the current study, we demonstrate that the broad specificity human PPT converts apo-FDH to holoenzyme and thus activates FDH catalysis."

"In our experiments, incubation of this enzyme with purified recombinant human PPT in the presence of CoA and Mg 2+ restored 10-fTHF dehydrogenase activity. Using a CoA-derived fluorescent reporter, we have also directly demonstrated that recombinant PPT can modify FDH in vitro . Phosphopantetheinylation by PPT occurs specifically at serine 354 of FDH because replacement of this serine with alanine prevented this covalent modification."

"Despite the lack of typical ALDH catalysis, mtFDH was able to perform the characteristic 10-fTHF dehydrogenase reaction after reactivation by recombinant 4'-phosphopantetheinyl transferase (PPT) in the presence of coenzyme A. Using site-directed mutagenesis, it was determined that PPT modifies mtFDH specifically at Ser375."

"Here, we describe a systematic map of ?14,000 high-quality human binary protein-protein interactions."

"With more than 56,000 candidate interactions, BioPlex 2.0 contains more than 29,000 previously unknown co-associations and provides functional insights into hundreds of poorly characterized proteins while enhancing network-based analyses of domain associations, subcellular localization, and co-complex formation."

"Here, we present HuRI, a systematically generated human protein interactome map with more than 50,000 PPIs of high biophysical quality. While HuRI displays highly significant overlap with known functional relationships, the cellular function of most individual PPIs remains to be elucidated."

"In combination with a quantitative chemical proteomic platform, we enriched and identified all the currently known 4'-phosphopantetheinylated proteins with high confidence, and unambiguously determined their exact sites of modification."

"Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins."

"We found that relative to control cells, PPT-deficient cells have reduced levels of both holo- and octanoyl-NDUFAB1 (Fig. 2F and G)."

"We observed significant Venus signal only when PPT-VN cells were co-expressed with either CS-VC or NDUFAB1-VC, indicating that PPT localizes to the mitochondrial matrix (Fig. 3F–H, see Supplementary Fig. 3G for gating strategy)."

"We found that all variants, except for PPT-GFP P12S, exhibited weaker rescue than wild type PPT (Fig. 5F–H, Supplementary Fig. 5G). Of note, cells expressing the PPT-GFP W79G variant showed low levels of protein expression compared to the other variants (Fig. 5F, Supplementary Fig. 5G–H) despite expression from the same promoter, suggesting that this variant may result in protein instability. These data identify R47H, A72T, W79G, R83C, and K96E as potential clinically observed pathologic variants of AASDHPPT, with molecular phenotypes attributable to altered mtFAS activity and perturbed cellular respiration."

"Cytosolic AASDHPPT (alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase) catalyzes the transfer of a phosphopantetheine moiety from coenzyme A to serine 2156 within the ACP domain of FAS (fatty acyl synthase)."

"Vitamin B5 ((R)-pantothenate, PanK), is an essential precursor for the synthesis of the metabolic cofactor Coenzyme A (CoA-SH) (Robishaw and Neely 1985) and is the prosthetic group of acyl carrier protein (ACP) (Joshi et al. 2003)."

"CC -!- FUNCTION: Catalyzes the post-translational modification of target CC proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine CC moiety from coenzyme A, regardless of whether the CoA is presented in CC the free thiol form or as an acetyl thioester, to a serine residue of a CC broad range of acceptors including the acyl carrier domain of FASN."

"CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:12815048}."