Five new genes are important for common polysaccharide antigen biosynthesis in Pseudomonas aeruginosa.
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pa5455 and pa5456 are putative glycosyltransferases by sequence analysis only.
"Gene sequence analysis indicated that pa5455 and pa5456 encode putative glycosyltransferases."
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Deletion of either gene abolishes CPA, and complementation restores it.
"Mutation of either one of the two putative glycosyltransferase genes, pa5455 and pa5456, completely abrogated CPA biosynthesis"
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The evidence is genetic; no enzyme assay is reported.
"We have presented evidence from genetic studies that the genes in the pa5455-pa5459 cluster are involved in CPA biosynthesis in P. aeruginosa."
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Co-overexpression shortens CPA chains; the authors speculate on a chain-terminating role.
"One or both of the proteins encoded by these genes is therefore the best candidate for the catalyst of the chain length-determining reaction."
Biosynthesis of the Common Polysaccharide Antigen of Pseudomonas aeruginosa PAO1: Characterization and Role of GDP-D-Rhamnose:GlcNAc/GalNAc-Diphosphate-Lipid α1,3-D-Rhamnosyltransferase WbpZ.
UniProt entry Q9HTB5 (Q9HTB5_PSEAE)
Annotation inferences using phylogenetic trees