Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
A primer-dependent polymerase function of pseudomonas aeruginosa ATP-dependent DNA ligase (LigD).
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Purified LigD has autonomous polymerase (533-840) and DNA ligase (188-527) domains.
"Here we purify LigD and show that it possesses an intrinsic polymerase function resident within an autonomous C-terminal polymerase domain, LigD-(533-840), that flanks an autonomous DNA ligase domain, LigD-(188-527)."
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The Pol domain extends primer-templates and adds non-templated nucleotides.
"(iii) templated extension of a 5'-tailed duplex DNA primer-template"
Novel 3'-ribonuclease and 3'-phosphatase activities of the bacterial non-homologous end-joining protein, DNA ligase D.
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The PE domain removes 3'-terminal ribonucleosides and then hydrolyses the 3'-phosphate.
"(i) the 3'-terminal nucleoside is first removed to yield a primer strand with a ribonucleoside 3'-PO4 terminus, and (ii) the 3'-PO4 is hydrolyzed to a 3'-OH"
Gap filling activities of Pseudomonas DNA ligase D (LigD) polymerase and functional interactions of LigD with the DNA end-binding Ku protein.
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Ku stimulates LigD and promotes plasmid end joining by full-length LigD.
"We find that Pseudomonas Ku stimulates POL-catalyzed ribonucleotide addition to a plasmid DSB end and promotes plasmid end joining by full-length Pseudomonas LigD."
UniProt entry Q9I1X7 (LIGD_PSEAE)