CRADD primary-source access and short excerpts

PMID:11821383

Original DOI: 10.1074/jbc.M107811200. Publicly indexed author-upload original, JBC page 13956, Figure 7C caption and the “CARD-8 Is an Apoptotic Protein” Results section. The indexed text, target Methods and caption were read; no downloaded PDF or figure-pixel inspection is claimed. The unchanged publication cache contains the abstract, which does not foreground the CRADD positive control.

Exact short caption excerpt: “vector or FADD, CRADD, Bcl-10, and CARD-8FL expression vectors.”

The caption describes MCF-7 cells cotransfected with GFP and the named expression vectors; dead cells were counted 36 hours later. This supports the CRADD positive-apoptosis annotation, not transfer of CARD8’s caspase-1 or NF-kappaB findings. Saved indexed-original SHA256: 9dfaf7a1fbf0e067c31f23ed2b2ec408160100241e22313794c33f4297031305.

Other original-source access

• PMID:8985253 original Nature PDF: printed pages 86–89; original binding Figure 4 distinguishes direct CASP2/RIP binding from bridged TNFR1/TRADD association. Independent consultation read the complete paper and inspected printed pages 87–89. Historical 200-residue construct numbering is distinct from the current 199-residue product.
• PMID:9044836(https://pubmed.ncbi.nlm.nih.gov/9044836/): publicly indexed author-upload original Methods/domain-binding/apoptosis Results and captions were recovered and independently read. Receptor involvement is proposed. No PDF pixels were inspected.
• PMID:19593445 complete original, original XML: complete Introduction, Methods, Results, Discussion and all three figure captions were examined. These describe BAD/prostate-cancer experiments, not a CRADD or mechanical-stimulus experiment. This is the basis for a citation-specific MISCITED judgment, not a biological impossibility claim.
• PMID:15073321(https://doi.org/10.1126/science.1095432): genuine complete abstract available; bounded original recovery did not yield full Methods/Results. Later sources are identified separately and are not silently substituted as this paper’s contents.
• PMID:17289572(https://pubmed.ncbi.nlm.nih.gov/17289572/), primary structural record 2OF5: human RAIDD94–199 and PIDD778–883 death-domain assembly. The deposited PIDD expression tail includes M plus AAALEHHHHHH; an unqualified “minimal His tag” description is inaccurate.

This authored file records access and citations; the linked original publications remain the biological evidence.

PMID:21726810

Original DOI10.1016/j.molcel.2011.06.012 and PMC3160283, Results on Figure6 and the PIDDosome requirement for MDM2 cleavage. The actual richer genuine XML extraction was read (paragraph48 in its saved index); the pre-existing shared repository cache lacks this Results passage and is preserved unchanged.

Exact short clauses from that Results paragraph: “inhibition of PIDD or RAIDD”; “reduction in Mdm2 cleavage”. The paragraph reports that the knockdowns reduce damage-induced MDM2 cleavage in human U2OS cells while sparing the pro-CASP3 control, and identifies CASP2 as the cleavage enzyme. This is target-specific evidence for adaptor participation in p53 reinforcement, not CRADD protease activity. Research capture SHA256: 3ff920e31a65ff077b4969bf3730b6d0b4544a93bb451d0d4067a0412aad3cc0.