Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for moeA
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UniProt assigns Q88L14 molybdopterin molybdenumtransferase activity, EC 2.10.1.1, producing Mo-molybdopterin from adenylyl-MPT and molybdate.
"CC -!- FUNCTION: Catalyzes the insertion of molybdate into adenylated
CC molybdopterin with the concomitant release of AMP.
CC {ECO:0000256|ARBA:ARBA00002901, ECO:0000256|RuleBase:RU365090}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP +
CC H(+); Xref=Rhea:RHEA:35047, ChEBI:CHEBI:15378, ChEBI:CHEBI:36264,
CC ChEBI:CHEBI:62727, ChEBI:CHEBI:71302, ChEBI:CHEBI:456215;
CC EC=2.10.1.1; Evidence={ECO:0000256|ARBA:ARBA00047317};"
UniProtKB entry P12281 (MOEA_ECOLI)
In vitro molybdenum ligation to molybdopterin using purified components.
Protein abundance profiling of the Escherichia coli cytosol.
OpenScientist gene research for moeA
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The report gives the conserved MoeA reaction used here.
"> **adenylyl-molybdopterin (MPT-AMP) + molybdate (MoO₄²⁻) → Mo-molybdopterin (Moco) + AMP + H⁺** (Mg²⁺-dependent)"
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The report explicitly identifies the lack of target-specific experimental evidence.
"- No *P. putida* KT2440-specific biochemical or structural study of PP_2123 was found; functional assignment relies on high-confidence orthology to *E. coli* MoeA and conserved domain architecture. Direct enzymatic characterization or a KT2440 knockout phenotype would confirm substrate specificity (Mo vs W) and downstream molybdoenzyme dependencies in this organism."
PANTHER representative entries for the MoeA/gephyrin family
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PTHR10192 contains bacterial MoeA proteins, including E. coli P12281.
"P12281,Molybdopterin molybdenumtransferase,protein,83333,Escherichia coli (strain K12),Escherichia coli (strain K12),moeA,411,PTHR10192:SF5,GEPHYRIN,True"