CYP79B2 (At4g39950, O81346) curation notes

Sources

• Falcon deep research (CYP79B2-deep-research-falcon.md), UniProt O81346, cached PMIDs.
• Added and cached: PMID:10922360 (Mikkelsen 2000), PMID:12464638 (Zhao 2002), PMID:15148388 (Glawischnig 2004), PMID:26352477 (Rajniak 2015). All verified via PubMed MCP.
• Abstract-only in cache: 10681464, 16167893, 17573535, 20023151, 20230487, 31511315, 10922360, 12464638, 15148388.

Core biochemistry

• Trp -> IAOx, EC 1.14.14.156 PMID:10922360; independently PMID:10681464. Km 21 uM.
• Electron donor is NADPH-P450 reductase (flavoprotein); Rhea uses reduced [NADPH--hemoprotein reductase]. In GO, GO:0090489 is_a GO:0016712 (flavoprotein donor), NOT GO:0016709 (NAD(P)H donor) -> MODIFY the IBA GO:0016709 to GO:0016712 (same as CYP79F1 review).

Which downstream processes does CYP79B2 actually catalyse a step of?

• Camalexin: yes, first step PMID:15148388; also physically in the ER metabolon with CYP71A13 PMID:31511315. ACCEPT GO:0010120.
• Indole glucosinolates: yes, first step PMID:10922360; PMID:15148388. GO:0009759 is NOT in GOA for CYP79B2. Comparator: CYP83B1 (next enzyme) carries GO:0009759 IDA; CYP81F2 IMP. So the term is applied to catalysing enzymes. Proposed via MODIFY of ARBA GO:0044272 sulfur compound biosynthetic process -> GO:0009759 (is_a descendant) rather than NEW.
• IAA: a step in a minor, conditional IAOx->IAN->IAA route PMID:15772288; KEEP_AS_NON_CORE.
• L-tryptophan catabolic process: Trp is consumed by the reaction; ACCEPT (not wrong, less informative).
• Defense rows (oomycete, bacterium, callose, ISR, generic defense): all necessity data from cyp79b2 cyp79b3; antimicrobial work done by downstream products (PEN2/CYP81F2 IGS hydrolysis, PAD3 camalexin). KEEP_AS_NON_CORE. Callose: context-dependent; Schlaeppi 2010 found WT-like callose in the double mutant with P. brassicae PMID:20230487.
• Notably GOA has no "defense response to fungus" row for CYP79B2 (project question 3); not proposed as NEW (necessity, not participation).

Location

• ER: CYP79B2-RFP ER-like, colocalizes with CYP71A13-GFP in N. benthamiana (deep research, Mucha 2019 Fig 6G-I). Chloroplast ISM -> REMOVE (AtSubP mis-call on N-terminal anchor). Membrane rows -> MODIFY to GO:0005789 ER membrane.

Drought / insect

• Drought: single transcript decrease PMID:23144921 -> MARK_AS_OVER_ANNOTATED (as for PAD3 review). Insect: aphid-induced expression -> KEEP_AS_NON_CORE.