"microscopic screening of 49 845 transformants yielded 6954 transformants which exhibited GFP fluorescence"

"a lack of O-mannosylation results in abnormal cell wall and septum formation, thereby severely affecting cell morphology and cell-cell separation"

"2887 distinct phosphorylation sites were identified from 1194 proteins with an estimated false-discovery rate of <0.5% at the peptide level"

"SPBC30B4.01cwsc1+Transmembrane receptor1PMZK373"

"Wsc1p-GFP was concentrated in patches at the cell tips, it interacted with the Rho-GEF Rgf2p"

"its overexpression activated cell wall biosynthesis"

"Each gene could be deleted individually without affecting viability, but the deletion of both was lethal and this phenotype was rescued by overexpression of the genes encoding either Rho1p or its GDP/GTP exchange factors (GEFs)"

"the second one implicates signaling from Wsc1p and Rgf2p through Rho1p to activate glucan synthase (GS)"

"wsc1Δ and mtl2Δ cells showed a low level of Rho1p-GTP under cell wall stress"

"This feedback was mediated by mechanosensing through the CW integrity pathway, which probes strain in the wall to adjust synthase localization and activity to surface growth ...Mutants defective in thickness homeostasis lysed by rupturing the wall"

"we uncovered the formation of micrometer-sized clusters at sites of force application onto the CW. Clusters assembled within minutes of CW compression, in dose dependence with mechanical stress and disassembled upon relaxation"

"Wsc1 accumulates to sites of enhanced mechanical stress through reduced lateral diffusivity, mediated by the binding of its extracellular WSC domain to CW polysaccharides, independent of canonical polarity, trafficking, and downstream CW regulatory pathways"

"Wsc1 may represent an autonomous module to detect and transduce local surface forces onto the CW"

"Wsc1 is a plasma-membrane/cell-surface, cell wall stress sensor-like protein** implicated in maintaining cell wall integrity under stress by coupling extracellular wall status to intracellular signaling"

"wsc1Δ** and **mtl2Δ** are individually viable, but **wsc1Δ mtl2Δ** is lethal; this lethality is **rescued by overexpression of Rho1 or its GEFs**, placing Wsc1/Mtl2 functionally upstream of Rho1 activation"

"Wsc1 **interacts with the Rho-GEF Rgf2**, and **Wsc1 overexpression activates cell wall biosynthesis**, consistent with a Wsc1 → Rgf2 → Rho1 axis"

"CIP/Pmk1 activity is not markedly reduced in their absence**, supporting partially MAPK-independent roles for these sensors"

"plasma membrane-associated, serine-rich cell wall “mechanosensor”**, localized at active growth sites and the division septum, proposed to detect mechanical stress associated with growth and wall remodeling"

"Wsc1 as a **mechanosensing module** that can form **stable clusters** acting as signaling platforms at sites of mechanical stress, feeding into Rho1-centered control of cell wall homeostasis"