Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
Cloning of human 3-hydroxyanthranilic acid dioxygenase in Escherichia coli: characterisation of the purified enzyme and its in vitro inhibition by Zn2+.
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Purified recombinant human 3-HAO converts 3-hydroxyanthranilic acid to quinolinic acid and is catalytically active only in the presence of Fe(II); Zn2+ inhibits the reaction.
"The recombinant human protein, obtained in E. coli, retains its enzymatic activity which can occur only in the presence of Fe(II)"
Towards a proteome-scale map of the human protein-protein interaction network.
Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.
A proteome-scale map of the human interactome network.
Crystal structures of human 3-hydroxyanthranilate 3,4-dioxygenase with native and non-native metals bound in the active site.
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Human 3HAO is a non-heme iron extradiol ring-cleaving dioxygenase; crystal structures show native iron bound in the active site.
"3HAO is a non-heme iron-containing, ring-cleaving extradiol dioxygenase that catalyzes the addition of both atoms of O2 to the kynurenine pathway metabolite 3-hydroxyanthranilic acid (3-HANA) to form quinolinic acid (QUIN)."
NAD Deficiency, Congenital Malformations, and Niacin Supplementation.
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Biallelic loss-of-function variants in HAAO (and KYNU) reduce enzyme activity, cause systemic NAD deficiency, and produce a congenital malformation syndrome; niacin supplementation prevents defects in mice.
"Variants were identified in two genes that encode enzymes of the kynurenine pathway, 3-hydroxyanthranilic acid 3,4-dioxygenase (HAAO) and kynureninase (KYNU)."
3-Hydroxyanthranilate oxygenase activity is increased in the brains of Huntington disease victims.
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3-hydroxyanthranilate oxygenase activity is detectable in human brain and is increased in the striatum of Huntington disease brains.
"The activity of 3-hydroxyanthranilate oxygenase is increased in Huntington disease brains as compared to control brains."
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Molecular cloning and functional expression of human 3-hydroxyanthranilic-acid dioxygenase.
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Cloning and functional expression of human 3-HAO; recombinant enzyme catalyzes quinolinate synthesis with Km(3-HANA) ~2 microM.
"The Km value of 3-HANA for recombinant h3-HAO (approximately 2 microM) was in good agreement with that reported for the native enzyme."
3-hydroxyanthranilate + O2 => 2-amino-3-carboxymuconate semialdehyde