The decision table contains 19 complete source-to-review joins across 13 database reference groups. The lane preserves all 37 imported source objects and their order, all three seeded splice products, the 19 original reference identities and every unowned review. No new annotation or supporting quotation is added.
The phylogeny evidence records the official PTHR23065 membership and five literal IBD rows. The official family label is PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1; human FCHO1 belongs to PTHR23065:SF6, F-BAR DOMAIN ONLY PROTEIN 1. A broad family label is not a claim that FCHO1 performs phosphatase chemistry. All five donor sets match the selected PAINT rows, including expected target self-WITH.
The original and single recovery requests for the target gene record, tree and family MSA timed out. No target leaf path or family alignment was verified. These access limits do not demonstrate divergence and donor count is not used to reject an ancestral assertion. Independent human target evidence in PMID:22484487 and PMID:32098969 supports membrane association, endocytic interactions and coat participation. Those studies also limit claims of universal obligatory nucleation or equivalent effects on every cargo.
The current ARBA00028399, ARBA00089731 and ARBA00044606 consequences were inspected, together with their Primates/FunFam condition sets. Exact target FunFam matching and historical rule state were not replayed. The combined coated-pit source also includes the actual UniProt SL-0069 field. Agreement among electronic pipelines is not an additional independent assay.
The actual HPA record reports enhanced nucleoplasm and cytosol evidence using HPA041653 and HPA062981. Their separate antigens match canonical residues 285–364 and 746–818. Three original composite images were inspected: both antibodies in MCF-7 and HPA062981 in PC-3. The recorded nuclear and extranuclear staining is retained, with cell-line differences in the cytosol call. Two other image requests failed. No independent endogenous specificity control, nuclear molecular function or isoform-specific localization was established. Nucleoplasm remains contextual; the endocytic membrane role does not negate another pool.
Cytosol is a direct part_of child of cytoplasm in the saved official ontology response. The broad IBA cytoplasm term is covered by the positive cytosol records and is marked TOO_GENERAL without asserting biological falsehood. Plasma membrane and clathrin-coated pit describe distinct retained locations; a pit is not synonymous with a free mature coated vesicle.
All eight source events reach actual human FCHO1 entity 8862257, reference entity 229459, O14526 residues 1–889, assigned to cytosol. The table supplies a complete sequence of event/component/member edges for each source. The PIK3C2A phosphorylation event reaches FCHO1 through catalyst activity 8868073 and complex 8868051. This establishes membership in the modeled assembly and does not assign PIK3C2A or AAK1 kinase chemistry to FCHO1.
The source explicitly models recruitment of the cytosolic FCHO1/FCHO2 set to the membrane and later release when other BAR proteins arrive. It states that the precise release timing is unknown. A nested protein compartment is a pathway model, not a separate localization assay; the independent HPA cytosol observation supports the retained target location. Only actual target routes were expanded, not every unrelated branch or cited experiment. The 2010 FCHo paper has an Expression of Concern and later Erratum whose scope is handled by the direct reference owner; neither a retraction nor universal invalidity is inferred here.
PMID:35072626 was read as the original rat hippocampal study, including Methods, relevant Results and Discussion, Figure 4/5 captions and actual Figure 4/5 pixels. Tagged FCHO1 appears at a subset of perisynaptic sites and can be transiently recruited. The stable position of the endocytic zone does not require every protein to reside there permanently. The Figure 5C comparison does not report a statistically significant FCHO1 difference; lifetime analysis and transient examples are separate observations. FCHO1 was not among the endogenous knock-in controls or later super-resolution component panel. Plasmid species was not independently resolved. Actual Ensembl one-to-one orthology, matching aligned protein sequences and the rat SynGO donor records support a bounded human inference, retained as non-core rather than direct human neuronal evidence.
PMID:41943152 supplies separate 2026 rat presynaptic endocytosis context. The complete original main text and figure captions were read, although its authentic normal cache remains abstract-only after an incomplete network read. No original figure pixels or supplements were inspected. Its reporter and domain-deletion limitations are explicit, and adaptor recruitment is inferred rather than directly measured. This source is not substituted for the postsynaptic localization experiment and does not create a new human neuronal annotation.
Selected original main-text passages and captions were independently read in the existing full caches for PMID:22484487 and PMID:32098969; their complete reference objects remain owned by the direct lane. Figure pixels, movies and supplements for those papers were not rereviewed here. GO_REF documents describe methods, not gene-specific experiments. The three official splice products remain exact. Tagged constructs, deletion mutants and disease variants are not made into natural products. No experimental-design recommendations are included in this database lane.